Table 1

M. tuberculosis strains and plasmids

Strains/plasmids

Characteristics

Source


E. coli DH5α

Invitrogen


M. tuberculosis H37Rv

wild-type laboratory strain

ATCC 25618


FAME1

M. tuberculosis suhBΔ

This study


FAME2

M. tuberculosis impAΔ

This study


FAME4

M. tuberculosis impCΔ::pFM96

This study


FAME7

M. tuberculosis::pFM54 (impCΔ SCO)

This study


FAME9

FAME7 ::pFM96

This study


FAME11

FAME7::pFM123

This study


FAME63

FAME7::FM203

This study


FAME5

M. tuberculosis ino1Δ

[23]


FAME12

M. tuberculosis ino1Δ::pFM54 (SCO)

This study


FAME35

M. tuberculosis::pFM151 (cysQΔ SCO)

This study


FAME43

FAME35::FM164

This study


FAME53

cysQΔ::FM164

This study


FAME87

FAME35::FM203

This study


FAME93

cysQΔ::FM203

This study


FAME 120

M. tuberculosis

cysQΔ:: pUC-Hyg-int

This study


pBluescript II SK+

Stratagene


pGEM5

Promega


pUC-Gm-int

pUC-based plasmid with HindIII cassette carrying gm and L5 int

[54]


pUC-Hyg-int

pUC-based plasmid with HindIII cassette carrying hyg and L5 int

[54]


p2NIL

gene manipulation vector, kan

[26]


pGOAL19

hyg pAg85-lacZ sacB PacI cassette vector

[26]


pIMP50

pGEM5::impA

This study


pIMP51

pGEM5::impAΔ (SphI 200 bp)

This study


pIMP57

p2NIL::impAΔ (SphI 200 bp)

This study


pFM74

p2NIL::impAΔ (769 bp)

This study


pFM75

pFM74 with PacI cassette of pGOAL19

This study


pFM33

p2NIL::suhB

This study


pFM48

pFM33::suhBΔ

This study


pFM52

pFM48 with PacI cassette of pGOAL19

This study


pFM31

p2NIL::impC

This study


pFM53

pFM31::impCΔ

This study


pFM54

pFM53 with PacI cassette of pGOAL19

This study


pFM94

pBluescript SK+::impC (+288 bp upstream)

This study


pFM96

pFM94::int gm

This study


pFM123

pFM96::impC D86N

This study


PMN013

plasmid carrying the M. smegmatis porin gene mspA

[44]


pFM203

pMN013::int gm

This study


pFM145

p2NIL::cysQ

This study


pFM148

pFM145::cysQΔ

This study


pFM151

pFM148 with PacI cassette of pGOAL19

This study


pFM160

pBluescript SK+::cysQ (+352 bp upstream)

This study


pFM164

pFM160::int gm

This study


Movahedzadeh et al. BMC Microbiology 2010 10:50   doi:10.1186/1471-2180-10-50

Open Data