Open Access Highly Accessed Research article

The capsule of Porphyromonas gingivalis reduces the immune response of human gingival fibroblasts

Jorg Brunner1, Nina Scheres1, Nawal B El Idrissi1, Dong M Deng1, Marja L Laine1, Arie J van Winkelhoff2 and Wim Crielaard1*

Author Affiliations

1 Department of Oral Microbiology, Academic Centre for Dentistry Amsterdam, Universiteit van Amsterdam and Vrije Universiteit, Amsterdam, The Netherlands

2 Center for Dentistry and Oral Hygiene, Department of Oral and Medical Microbiology, University Medical Center Groningen, Groningen, The Netherlands

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BMC Microbiology 2010, 10:5  doi:10.1186/1471-2180-10-5

Published: 11 January 2010

Abstract

Background

Periodontitis is a bacterial infection of the periodontal tissues. The Gram-negative anaerobic bacterium Porphyromonas gingivalis is considered a major causative agent. One of the virulence factors of P. gingivalis is capsular polysaccharide (CPS). Non-encapsulated strains have been shown to be less virulent in mouse models than encapsulated strains.

Results

To examine the role of the CPS in host-pathogen interactions we constructed an insertional isogenic P. gingivalis knockout in the epimerase-coding gene epsC that is located at the end of the CPS biosynthesis locus. This mutant was subsequently shown to be non-encapsulated. K1 capsule biosynthesis could be restored by in trans expression of an intact epsC gene. We used the epsC mutant, the W83 wild type strain and the complemented mutant to challenge human gingival fibroblasts to examine the immune response by quantification of IL-1β, IL-6 and IL-8 transcription levels. For each of the cytokines significantly higher expression levels were found when fibroblasts were challenged with the epsC mutant compared to those challenged with the W83 wild type, ranging from two times higher for IL-1β to five times higher for IL-8.

Conclusions

These experiments provide the first evidence that P. gingivalis CPS acts as an interface between the pathogen and the host that may reduce the host's pro-inflammatory immune response. The higher virulence of encapsulated strains may be caused by this phenomenon which enables the bacteria to evade the immune system.