Figure 5.

Overexpression of MAT1-1-1 and BEM1 in G217B. A: Detection of c-myc tagged recombinant fusion protein using anti-c-myc antisera on a Western blot of homogenates of H. capsulatum strains overexpressing Bem1 (lane 2), Mat1-1-1 (lane 5) or a control strain (lane 1). Detection of HSP60 as a loading control is shown on a duplicate blot in lane 3 and lane 4. B: RNA levels of MAT1-1-1 in mycelial phase G217B (n = 4), UC1 (n = 6), and UC26 (n = 4) compared to levels in strains overexpressing MAT1-1-1 and BEM1 in the G217B background (n = 3) # = below level of detection. C: RNA levels of PPG1 in mycelial phase G217B (n = 4), UC1 (n = 7), and UC26 (n = 4) compared to levels in strains overexpressing MAT1-1-1 and BEM1 in the G217B background (n = 3). *** = p ≤ 0.001.

Laskowski and Smulian BMC Microbiology 2010 10:49   doi:10.1186/1471-2180-10-49
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