Effect of mAb MEST-3 on yeast formation. P. brasiliensis hyphae fragments were suspended in 1 ml of PGY medium and supplemented or not with mAb MEST-3 (50 μg/ml). Cells were placed on a 24-well plate at 37°C, and after 96 h of incubation, hyphae differentiation into yeast (M→Y) forms was observed under microscope. Panel A shows M→Y differentiation in free-mAb medium, Panel B shows M→Y differentiation in medium containing MEST-3, and Panel C shows the mycelia growth of hyphae fragments maintained at 23°C for 96 h in free-mAb medium.
Toledo et al. BMC Microbiology 2010 10:47 doi:10.1186/1471-2180-10-47