Effect of monoclonal antibodies on yeast to mycelium transformation. Yeast forms of P. brasiliensis, H. capsulatum and S. schenckii were incubated for one hour with different concentration of MEST-1, -2 and -3, and control IgG at 37°C. After that the yeast cultures were transferred to a 25°C incubator, and kept for 2 days. Three hundred yeasts were counted, and the number of yeast showing hyphae growth was evaluated. In control experiment 100% of yeast showed hyphae formation; the results represent the percentage of those incubated with an irrelevant mAb, considered as 100% of yeast to mycelium transformation. MEST-1 (closed square), MEST-2 (closed circle) and MEST-3 (closed triangle). * p < 0.05.
Toledo et al. BMC Microbiology 2010 10:47 doi:10.1186/1471-2180-10-47