Effect of monoclonal antibodies on fungal growth. Panel A, Yeast forms of P. brasiliensis, H. capsulatum and S. schenckii were incubated for 24 h with mAbs, or a control IgG or left alone, at 37°C. Yeasts were transferred to a petri dish containing PGY or BHI-agar medium, and incubated for 2 days at 37°C. Colony forming units (CFUs) were counted, and expressed as percentage of those incubated with an irrelevant mAb, considered as 100% of CFU. Panel B, MTT assay of fungi after incubation with mAbs MEST-1, -2, and -3. Yeast forms of P. brasiliensis, H. capsulatum and S. schenckii were incubated with mAbs, a control IgG or left alone. After incubation for 48 h, MTT solution was added to each well and was further incubated for 3 h at 37°C. The absorption was measured at 580 nm. MEST-1 (closed square), MEST-2 (closed circle) and MEST-3 (closed triangle). * p < 0.05.
Toledo et al. BMC Microbiology 2010 10:47 doi:10.1186/1471-2180-10-47