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Open Access Research article

Elucidation of the outer membrane proteome of Salmonella enterica serovar Typhimurium utilising a lipid-based protein immobilization technique

Darren Chooneea1*, Roger Karlsson2, Vesela Encheva4, Cath Arnold1, Hazel Appleton3 and Haroun Shah1

Author Affiliations

1 Department for Bioanalysis and Horizon Technologies, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK

2 Department of Chemistry, University of Gothenburg, Kemivägen 10 SE-412 96 Göteborg, Sweden

3 Virus Reference Department, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK

4 Laboratory of the Government Chemist, Queens Rd, Teddington, Middlesex, TW11 0LY, UK

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BMC Microbiology 2010, 10:44  doi:10.1186/1471-2180-10-44

Published: 11 February 2010

Additional files

Additional file 1:

Outer membrane proteins identified and number of peptides generated using a single or multi-step digest protocol. Table listing outer membrane proteins identified from single and multi-step digest protocols after using the LPI™ FlowCell

Format: DOC Size: 141KB Download file

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Additional file 2:

Comparison of the outer membrane proteins identified in this study with that reported by Coldham & Woodward and Molloy et al. Table comparing the results from this study with that reported by Coldham & Woodward and Molloy et al.

Format: DOC Size: 115KB Download file

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Additional file 3:

Flow diagram showing the basic steps in operating a LPI™ FlowCell. A flow diagram showing the main steps in using the LPI™ FlowC

Format: DOC Size: 35KB Download file

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Open Data