Specific activities of the quinone-dependent D-lactate dehydrogenase Dld (A) and growth (B) of various C. glutamicum strains. Specific Dld activities (A) were determined after growth in LB complex medium containing 1 mM IPTG. The values represent means and standard deviations of at least three independent cultivations. Growth (B) of C. glutamicum WT(pEKEx3) (diamonds), WT(pEKEx3-dld) (circles), ::dld(pEKEx3) (squares), and ::dld(pEKEx3-dld) (triangles) in CgXII mineral medium containing 100 mM D-lactate and 1 mM IPTG was monitored as OD600nm (open symbols). The concentration of D-lactate in the supernatant was measured by HPLC (closed symbols). Averages and experimental errors from at least three independent growth experiments are shown.
Kato et al. BMC Microbiology 2010 10:321 doi:10.1186/1471-2180-10-321