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Open Access Highly Accessed Research article

Genes regulated by the Escherichia coli SOS repressor LexA exhibit heterogenous expression

Simona Kamenšek1, Zdravko Podlesek1, Osnat Gillor2 and Darja Žgur-Bertok1*

Author Affiliations

1 Dept. of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, Slovenia

2 Zuckerberg Institute for Water Research, J. Blaustein Institutes for Desert Research, Ben-Gurion University, Israel

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BMC Microbiology 2010, 10:283  doi:10.1186/1471-2180-10-283


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2180/10/283


Received:16 July 2010
Accepted:11 November 2010
Published:11 November 2010

© 2010 Kamenšek et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Phenotypic heterogeneity may ensure that a small fraction of a population survives environmental perturbations or may result in lysis in a subpopulation, to increase the survival of siblings. Genes involved in DNA repair and population dynamics play key roles in rapid responses to environmental conditions. In Escherichia coli the transcriptional repressor LexA controls a coordinated cellular response to DNA damage designated the SOS response. Expression of LexA regulated genes, e.g. colicin encoding genes, recA, lexA and umuDC, was examined utilizing transcription fusions with the promoterless gfp at the single cell level.

Results

The investigated LexA regulated genes exhibited heterogeneity, as only in a small fraction of the population more intense fluorescence was observed. Unlike recA and lexA, the pore forming and nuclease colicin activity genes as well as umuDC, exhibited no basal level activity. However, in a lexA defective strain high level expression of the gene fusions was observed in the large majority of the cells. All of the investigated genes were expressed in a recA defective strain, albeit at lower levels, revealing expression in the absence of a spontaneous SOS response. In addition, the simultaneous expression of cka, encoding the pore forming colicin K, and lexA, investigated at the single cell level revealed high level expression of only cka in rare individual cells.

Conclusion

LexA regulated genes exhibit phenotypic heterogeneity as high level expression is observed in only a small subpopulation of cells. Heterogenous expression is established primarily by stochastic factors and the binding affinity of LexA to SOS boxes.

Background

Genetically identical bacterial cells can exhibit heterogeneity as the population bifurcates into distinct subpopulations. Such heterogeneity within clonal populations is a bet hedging strategy as a small fraction of a population is either prepared to survive adverse environmental conditions or sacrifice itself to enhance the likelihood of survival of clonal siblings. Examples of phenotypic heterogeneity include: development of competence and sporulation in Bacillus subtilis, lysogenic versus the lytic cycle of bacteriophage lambda, biofilm formation, toxin production and antibiotic persistence [1-4].

In Escherichia coli DNA damage induces the expression of more than 40 genes leading to arrest of cell division and the induction of DNA repair, prophages, toxin production and mutagenesis [5]. The key regulator of this coordinated cellular response, named the SOS response [6], is the LexA protein that represses gene expression by binding as a dimer to a 16-mer consensus sequence CTG-N10-CAG designated as SOS boxes [7]. Upon DNA damage replication forks are stalled exposing single-stranded DNA (ssDNA). RecA binds to the ssDNA forming a nucleoprotein filament which activates the autoproteolytic cleavage of LexA, leading to induction of the SOS response. In addition to activation by exogenous DNA damaging agents, the SOS response is also induced by endogenous, as well as spontaneous events [5].

SOS induction often results in the production of antimicrobial toxins such as bacteriocins. The bacteriocins of E. coli strains, designated as colicins, are plasmid encoded and are found with high frequency among natural isolates [8]. These toxins were suggested to promote phenotypic and genotypic diversity within E. coli populations in the mammalian colon [9,10]. Colicins destroy cells by one of three mechanisms: (i) they either form pores in the cytoplasmic membrane thus depleting its electrochemical potential, (ii) degrade either the DNA or RNA of their target cell or (iii) inhibit peptidoglycan and lipopolysaccharide (LPS) O-antigen biosynthesis [11-13]. Production and release of most colicins is encoded by three genes, an activity gene encoding the colicin protein, an immunity gene encoding a protein that protects the cell from its produced toxin and a lysis gene for semispecific release of the colicin. Colicin encoding genes characteristically have two overlapping SOS boxes that bind two LexA dimers and protect the cell from untimely colicin production, as it is lethal to the producing cell [14]. Some colicins, such as colicins B and M, have no lysis genes and are actively secreted by an unknown mechanism [15]. Colicin B and M encoding operons are tightly linked on large conjugative plasmids [16,17]. Expression of both colicin B and colicin M seems to be regulated by a common SOS boxes located upstream of the colicin B activity gene [16,18].

In previous studies we showed that the pore forming colicin K activity gene cka is expressed in only a small fraction of a bacterial population while the immunity gene encoding the immunity protein is expressed in the large majority of the cells [3,19]. In the present study we investigated, at the single cell level, expression of the activity genes of several other colicins namely, the pore formers A, E1 and N, the DNase colicin E7 and the LPS synthesis inhibitor, colicin M. We compared the single cell colicin expression to the expression of other LexA regulated genes, e.g. recA, lexA and umuDC, and finally we examined the simultaneous expression of the colicin encoding cka gene and the lexA gene.

Methods

Bacterial strains, plasmids and growth conditions

The bacterial strains and plasmids used in this study are presented in Table 1. Bacteria were grown in Luria-Bertani (LB) with aeration at 37°C and with the appropriate antibiotics. Ampicillin and kanamycin (Sigma, St Louis MO, USA) were used at concentrations 100 μg ml-1 and 30 μg l-1, respectively.

Table 1. E. coli strains and plasmids used in the presented study

General DNA techniques

Plasmid DNA isolation was performed with the GeneJET™ plasmid miniprep kit (Fermentas, Burlington, Canada). Standard procedures were used for gel electrophoresis, ligations and transformation experiments [20]. Restriction endonuclease digestion was performed according to the instructions of the manufacturer (Fermentas). The PCR amplified fragments were purified using the QIAquick PCR purification kit (Qiagen, Hamburg, Germany). DNA fragments were isolated from agarose gels by using a QIAquick gel extraction kit (Qiagen).

Construction of promoter fusions

PCR was carried out to amplify the promoter regions with an additional 73 - 93 bp of the flanking colicin encoding gene for colicins A (486 bp), E1 (508 bp), E7 (501 bp), N (499 bp) and M (298 bp) with the primers listed in Table 2. All primers have added BamHI and XhoI restriction sites. The PCR generated fragments were cut with BamHI and XhoI (Fermentas), and ligated into the low copy number pSC101 [21] based plasmid with a promoterless (GFPmut3) gfp also cut with the same two enzymes.

Table 2. Primers used in this study

The promoterless DsRed-Express2 gene, which is part of a gene cassette on plasmid pDsRed-Express2-N1, was cloned into the natural colicin K encoding plasmid pColK-K235 manipulated to carry the Apr gene as a selectable marker, and a KpnI restriction site in the cka gene. A cassette carrying the promoterless gfp was inserted at the KpnI restriction site [19]. The cka-DsRed-Express2 transcriptional fusion was constructed by replacing the KpnI fragment harboring the promoterless gfp with the in frame promoterless DsRed-Express2 [22] amplified by PCR (Table 2) which was prior to ligation also cut with KpnI. Only cells with an active cka promoter can express DsRed-Express2.

Nucleotide sequencing was performed to confirm that no base changes had occurred during amplification. The sequences have been deposited in the GenBank Nucleotide sequence database under accession numbers, HM449002 (caa promoter region), HM449003 (cna promoter region), HM449004 (ce1a promoter region), HM449005 (ce7a promoter region), HM449006 (cma promoter region).

Fluorescence microscopy

Strains RW118 and RW464 carrying different colicin promoter region-gfp transcriptional fusions, and control strains without plasmid carrying gfp fusions, were grown with aeration at 37°C. Samples were removed at early stationary phase and chloramphenicol (500 μg ml-1) (Sigma) was added to block protein synthesis. Prior to microscopy, cells were attached to glass slides coated with 0.1% (wt vol-1) poly-L-lysine (Sigma). Fluorescence microscopy to detect expression in single cells was performed using an inverted microscope (Nikon Eclipse TE300), equipped with a Nikon digital camera DXM 1200, and a 488 nm Argon-Ion laser as well as bright field microscopy.

The examined cells were counted with software for quantification of bacteria by automated image analysis cellC http://www.cs.tut.fi/sgn/csb/cellc/ webcite. The fluorescence intensity of individual cells was estimated using image analysis software Scion Image http://www.scioncorp.com webcite as previously described [3]. The fluorescent micrographs were converted to greyscale images. The density window was established by using density slice matching the shape of the cells with the highest fluorescence intensity and that of the cells with the lowest intensity, gaining the top and the bottom boundaries (respectively) of the density window. For greater clearness the density index scale is determined from 0 (black) to 256 (white). All micrographs were taken at exactly the same conditions; thus the density window gives good correlation to the fluorescence intensity of the analyzed population.

Simultaneous expression of the cka-DsRed-Express2 and the lexA-gfp fusions was investigated employing a laser scanning Confocal Microscope (Zeiss, Göttingen, Germany).

Results and discussion

Pore forming and nuclease colicins exhibit heterogeneity

The advent of methods for visualization of gene expression in individual cells has revealed within populations of genetically identical bacteria heterogeneity in expression of certain genes [1-3]. A classical example of heterogeneity is the expression of the cka gene, encoding the pore forming colicin K; in the absence of exogenous DNA damaging agents cka is expressed in only a small fraction of the population [3,19] as the producing cells lyse to release the colicin. While colicin expression is characteristically regulated by the LexA protein which binds to overlapping SOS boxes, their regulatory sequences including SOS boxes are not identical. Therefore, we investigated at the single cell level, expression of genes encoding three pore formers (A, E1, N), a DNase colicin (E7) and a LPS inhibitor (colicin M), employing gfp transcriptional fusions. Our results revealed that, as was previously shown for the cka gene [19], only a small portion of the population expressed the investigated activity genes (colicin A, caa, Figure 1, Figure 2 and Table 3). We showed that single cell expression of these genes correlates with the predicted affinity of binding of the LexA protein to the operator sequences (Table 3), as expressed by the heterology index (HI). The HI was defined to determine the degree of divergence of any 20 nucleotide sequences from the consensus LexA-binding site [23]. Sequences with a low HI are closer to the consensus and are predicted to bind LexA with greater affinity than sites with a higher HI. Thus, the colicin E7 SOS boxes, which have the highest HI values and therefore the lowest predicted affinity of LexA binding, exhibit approximately three fold higher percentage of cells expressing the colicin activity gene compared to the pore forming colicins examined in this study. On the other hand, single cell analysis of cells harboring a gfp fusion with the colicin M activity gene promoter, cma-gfp, revealed low level expression in the large majority of the investigated cells. Colicin M was shown to be tightly connected with the upstream colicin B encoding genes and it is presumed that expression of both colicins B and M is regulated from common SOS boxes situated upstream of the colicin B activity gene [16,18]. Colicins M and B are among the most abundant colicins produced by E. coli strains [24]. We analysed the nucleotide sequences upstream of cma and found neither colicin regulatory motifs nor any consensus promoter sequence (data not presented). Nonetheless, we detected uniform low-level fluorescence mediated by the colicin M promoter (Figure 2, Table 3).

thumbnailFigure 1. Merged image of the phase contrast and fluorescence images of RW118 with a caa-gfp transcriptional fusion. Only a small subpopulation of cells exhibited high fluorescence intensity, while the large majority of the cells exhibited no fluorescence.

thumbnailFigure 2. Quantification of fluorescence intensity among strains expressing gfp transcriptional fusions. Number of cells from digital micrographs were calculated and to each cell the relative fluorescence was assigned with the use of Scion Image software. The average fluorescence value and number of cells within a narrow interval was plotted. A: Expression of gfp transcriptional fusions in RW118 and B: Expression in isogenic recA defective RW464.

Table 3. Cells expressing SOS regulated genes in the wild type RW118

SOS genes exhibit heterogeneity

Previously, single cell expression of a sulA-gfp fusion was investigated [25]. SulA is synthesized in large amounts during the SOS response and inhibits cell division by binding to FtsZ, the major component of the cell division machinery [26]. The sulA operator has a HI of 4.65 and thus binds LexA tightly. The authors found that in the absence of exogenous DNA damaging agents only approximately 0.3% of the examined cells fully expressed sulA.

As RecA is required to initiate the SOS response and LexA to repress the response, both are expressed, albeit at a low level, in the absence of DNA damage. A previous study showed a temporal program of expression of SOS genes upon DNA damage [21]. Subsequently, the response of individual cells to UV irradiation was followed by monitoring the activity of LexA repressed promoters fused to the promoterless gfp [27]. The authors found that the response is highly structured as several peaks in promoter activity were observed following DNA damaging UV irradiation. In our study we analyzed at the single cell level, the expression of the recA, lexA, and umuDC genes under physiological conditions using promoter fusions described previously [21]. Fluorescence microscopy revealed heterogeneity in the expression of all three genes. Based on fluorescence intensity, we found that the expression of recA (Figure 3) and lexA was high in a small percentage of the cells, 3.1 and 1.5%, respectively (Figure 2 and Table 3). In strains harboring the pore formers and DNase colicins transcriptional fusions to the gfp gene, heterogeneity was exhibited as a small subpopulation of highly expressing cells within the large majority of non-expressing cells. On the other hand, among the recA-gfp and lexA-gfp encoding populations, a small fraction exhibited high expression while the large majority exhibited basal level expression. The number of highly fluorescent cells harboring the recA-gfp fusion and their fluorescence intensity were higher compared with cells hosting lexA-gfp. The HI of the recA SOS box is lower than of the lexA, predicting a higher affinity of LexA binding however, lexA harbors two SOS boxes. These results are in agreement with the higher basal level of the RecA protein compared to LexA, 7,200 versus 1,300 protein molecules per cell, respectively [28]. The higher levels of RecA protein could be explained by its roles in the SOS response, homologous recombination and its involvement in other repair mechanisms such as recombinational repair.

thumbnailFigure 3. Merged images of the phase contrast and fluorescence images of recA-gfp expression. A: Expression of recA-gfp gene fusion in RW118 and B: Expression of recA-gfp fusion in the isogenic recA defective RW464 showing heterogeneity in expression of the recA gene.

The SOS genes, polB, dinB and umuDC, encode specialized DNA polymerases II, IV and V respectively, which can bypass DNA lesions yet with reduced fidelity introducing mutations to newly synthesized DNA [29]. The operon encoding PolV is regulated by an SOS box that exhibits one of the highest predicted LexA binding affinities, with a HI of 2.77 [30]. It was shown that only upon full induction of the SOS response UmuD is synthesized and persists as a full length dimer [31]. Accordingly, fluorescence emission from the umuDC-gfp fusion was observed in a very small fraction of the examined cells (0.09%) and no detectable basal level of expression was observed among the large majority of the population. Our results show that in the absence of exogenous DNA damaging agents very low levels of umuDC promoter activity is detected. As translesion synthesis must be employed only when necessary, synthesis of the specialized polymerases is under physiological conditions controlled by complex regulation at the level of transcription and posttranslation.

SOS regulated genes are expressed in a recA defective strain

Besides regulating DNA repair, induction of the SOS response has been recently shown to have a much broader role including, regulation of virulence factor synthesis in Staphylococcus aureus [31], type III secretion in enteropathogenic E. coli [32], subversion of innate defenses during urinary tract infection [33] and persistence to the fluoroquinolone antibiotic ciprofloxacin [34]. To examine to what extent expression of the investigated pore forming and DNase colicin activity genes as well as the recA, lexA and umuDC genes is dependent upon an SOS response under physiological conditions, expression was investigated in an isogenic recA defective strain RW464 (Figure 2). Analysis at the single cell level revealed reduction in the level of fluorescence and the number of intensely expressing cells harboring the recA, lexA, umuDC, caa as well as ce7a - gfp gene fusions and lower fluorescence of cells harboring ce1a and cna-gfp fusions. A greater reduction in the number of intensely expressing cells for colicin A (approximately ten fold) and colicin E7 (approximately three fold) was observed, while the percentage of cells expressing colicin E1 and N activity genes remained essentially unaltered (Figure 2, Table 4). The majority of the E. coli LexA regulon promoters are simple, being regulated only by a single transcriptional factor [35] however, some colicin encoding genes have additional regulation. Indeed, the CRP-cAMP complex was shown to stimulate expression of the colicin E1 activity gene ce1a by binding upstream from the ce1a SOS operator [36,37]. Interestingly, analysis of the colicin N promoter revealed a similar CRP binding site at the same location (Ghazaryan L., personal communication).

Table 4. Cells expressing SOS regulated genes in the recA defective strain RW464

Both the recA-gfp and lexA-gfp fusions were expressed in the recA defective strain RW464, albeit at a lower level compared to the wild type (Table 4, Figure 2, Figure 3), with a small fraction of the population exhibiting high fluorescence indicating that, stochastic factors could be involved. Filamentation due to delay in cell division is evident among the less robust recA defective strain. However, expression of the investigated genes was not limited to filamented cells (Figure 3).

To resolve the effect of LexA regulation at the single cell level, expression of the investigated gene fusions was also studied in strain RW542 encoding a LexA protein defective in binding to LexA boxes. Fluorescence microscopy revealed that in the lexA defective strain all cells harboring the lexA-gfp or recA-gfp fusions, as well as the large majority (98%) of the cells harboring gfp fusions with the colicin activity genes were intensely fluorescent, indicating high level expression (data not shown).

Simultaneous expression of the cka and SOS genes

The advent of novel fluorescence markers enables analysis of simultaneous expression of two or more genes. To investigate in detail how the expression of colicin genes correlates with the expression of SOS genes, simultaneous expression of the cka and the lexA genes was followed at the single cell level in strain RW118 harboring two plasmids: pKCT10 with a cka-DsRed-Express2 fusion and the pSC101 derivative vector harboring the lexA-gfp fusion. As is evident from Figure 4, the large majority of cells that more highly expressed the lexA gene also expressed the cka gene. Nonetheless, individual cells (approximately 0.1%) highly expressing only the cka gene could be detected suggesting, that in a very small fraction of the population the colicin K activity gene is expressed in the absence of the SOS response most probably stochastically, due to perhaps intracellular fluctuations of the LexA protein. Filamentation while a hallmark of SOS induction due to binding of SulA to the FtsZ proteins is also evident in cells not expressing lexA-gfp (Figure 4). Multimers of the natural cka-gfp encoding plasmid could be responsible for filamentation in the absence of SOS induction [38].

thumbnailFigure 4. Fluorescence images showing simultaneous expression of the cka-DsRed-Express2 and lexA-gfp transcriptional fusions. A:. Expression of cka-DsRed-Express2 gene fusion. B: Expression of lexA-gfp gene fusion in RW118. The arrow indicates a cell with high level expression of cka, but not lexA, showing that colicin activity gene expression can occur in the absence of the SOS response.

In conclusion, to our knowledge this is the first study exploring a number of SOS regulated genes at the single cell level under physiological condition. Exposure of a population of bacterial cells to a DNA damaging agent induces the SOS response in all susceptible cells. However, under physiological conditions, genes regulated by the LexA protein also exhibit heterogenous expression. We show that genes with a very high affinity of LexA binding, characteristic of overlapping SOS boxes of colicin operators, or very low HI such as umuDC, are expressed in only a small fraction of the population and exhibit no detectable basal level expression. In contrast, genes of the SOS regulon with a somewhat lower predicted affinity of LexA binding, such as lexA and recA, while also fully expressed in a small subpopulation, exhibit basal level expression. Intense fluorescence of cells harboring the investigated gene fusions was observed in a lexA defective strain indicating that the LexA protein effectively represses promoter activity in the large majority of cells. Some of the examined cells could be experiencing disruption of replication forks during replication and thus induction of the SOS response. However, expression of all of the investigated genes was observed in a recA mutant, which cannot instigate an SOS response indicating that, expression of LexA regulated genes also occurs stochastically.

Expression of colicin genes under physiological conditions by a small subpopulation may promote strain and genetic diversity and due to lysis of producing cells could provide resources to facilitate growth of non-expressing cells. On the other hand, a subpopulation of cells with higher levels of the RecA protein may be more proficient in recombination, e.g. for the stable incorporation of horizontally acquired DNA or a rapid response to DNA damage. We can speculate that heterogeneity of expression of lexA in E. coli affects a number of phenomenon significant for antibiotic tolerance/resistance (persisters), horizontal gene transfer (induction of prophage) and virulence among pathogenic E. coli strains. The same might apply to other gram negative (e.g. Shigella, Salmonella, Pseudomonas aeruginosa) and gram positive (e.g. S. aureus, B. subtilis) bacterial species that possess a system similar to the E. coli SOS system.

Conclusion

LexA regulated SOS genes exhibit heterogeneity as they are highly expressed in only a small subpopulation of cells. Unlike recA and lexA, the colicin activity genes and umuDC exhibit no basal level expression. Heterogenous expression is established primarily by stochastic factors as well as the binding affinity of LexA to SOS boxes.

Authors' contributions

SK performed all experiments. ZP contributed to analysis of the results. OG and DŽB participated in the design of the experiments and SK, OG and DŽB in preparation of the manuscript. All authors read and approved the final manuscript.

Acknowledgements

We thank Ben Glick for generously providing pDsRed-Express2-N1 as well as Uri Alon for strains carrying the lexA-gfp, recA-gfp and umuDC-gfp fusions. This work was funded by grant P1-0198 from the Slovenian Research Agency (ARRS) and the FP6 Sixth EU framework programme for Research and Technological Development: Transnational Access, Dryland Research Specific Support Action (SSA), Jacob Blaustein Institutes of Desert Research, Ben Gurion University of the Negev.

Simona Kamenšek is a recipient of a Ph.D grant from ARRS.

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