Table 3

Bacterial strains and plasmids used in the present study

Strain or plasmid

Relevant feature(s)

Source or reference


Strains

Acinetobacter baumannii

ATCC 17978

Wild-type strain

Laboratory stock

omp33::TOPO

Derived from ATCC 17978. omp33 mutant obtained by plasmid insertion. KanR, ZeoR

Present study

Δomp33::Km

Derived from ATCC 17978. omp33 mutant obtained by gene replacement. KanR

Present study

ΔoxyR::Km

Derived from ATCC 17978. oxyR mutant obtained by gene replacement. KanR

Present study

ΔsoxR::Km

Derived from ATCC 17978. soxR mutant obtained by gene replacement. KanR

Present study

ΔoxyR::Km-omp33::TOPO

Derived from ΔoxyR::Km. oxyR omp33 double mutant. KanR, ZeoR

Present study

ΔsoxR::Km-omp33::TOPO

Derived from ΔsoxR::Km. soxR omp33 double mutant. KanR, ZeoR

Present study

Escherichia coli

TG1

supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK- mK-) [F' traD36 proAB lacIqZΔM15]

Laboratory stock

Plasmids

pCR-BluntII-TOPO

Suicide plasmid for A. baumannii. KanR, ZeoR

Invitrogen

pTOPO33int

pCR-BluntII-TOPO containing a 387-pb internal fragment of the omp33 gene. KanR, ZeoR

Present study

pET-RA

A. baumannii replication origin. CTX-M14 β-lactamase gene promoter. RifR

Present study

pET-RA-OMP33

pET-RA containing the omp33 gene without its promoter region. RifR

Present study


Kan, kanamycin; Zeo, zeocin; Rif, rifampicin

Aranda et al. BMC Microbiology 2010 10:279   doi:10.1186/1471-2180-10-279

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