Open Access Highly Accessed Research article

Differential phenotyping of Brucella species using a newly developed semi-automated metabolic system

Sascha Al Dahouk12*, Holger C Scholz3, Herbert Tomaso4, Peter Bahn1, Cornelia Göllner1, Wolfram Karges2, Bernd Appel1, Andreas Hensel1, Heinrich Neubauer4 and Karsten Nöckler1

Author Affiliations

1 Federal Institute for Risk Assessment, Diedersdorfer Weg 1, D-12277 Berlin, Germany

2 RWTH Aachen University, Department of Internal Medicine III, Pauwelsstraße 30, D-52074 Aachen, Germany

3 Bundeswehr Institute of Microbiology, Department of Bacteriology, Neuherbergstraße 11, D-80937 Munich, Germany

4 Friedrich Loeffler Institute, Institute of Bacterial Infections and Zoonoses, Naumburgerstraße 96a, D-07743 Jena, Germany

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BMC Microbiology 2010, 10:269  doi:10.1186/1471-2180-10-269

Published: 23 October 2010

Additional files

Additional file 1:

List of biochemical reactions tested with the Taxa Profile™ A plate. The Taxa Profile™ A microtiter plate allows testing of 191 different amines, amides, amino acids, other organic acids and heterocyclic and aromatic substrates.

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Additional file 2:

List of biochemical reactions tested with the Taxa Profile™ C plate. The Taxa Profile™ C microtiter plate enables the analysis of 191 different mono-, di-, tri- and polysaccharides and sugar derivates.

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Additional file 3:

List of biochemical reactions tested with the Taxa Profile™ E plate. The Taxa Profile™ E microtiter plate is configured to determine the enzymatic activity of 95 amino peptidases and proteases, 76 glycosidases, phosphatases and other esterases, and also includes 17 classic reactions.

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Additional file 4:

Cluster analysis of Brucella reference and field strains based on their amino acid metabolism. Cluster analysis of 83 Brucella and 2 Ochrobactrum strains based on 191 biochemical reactions tested with the Taxa Profile™ A plate. Hierarchical cluster analysis was performed by the Ward's linkage algorithm using the raw OD data.

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Additional file 5:

Cluster analysis of Brucella reference and field strains based on their carbohydrate metabolism. Cluster analysis of 83 Brucella and 2 Ochrobactrum strains based on 191 biochemical reactions tested with the Taxa Profile™ C plate. Hierarchical cluster analysis was performed by the Ward's linkage algorithm using the raw OD data.

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Additional file 6:

Cluster analysis of Brucella reference and field strains based on specific enzymatic reactions. Cluster analysis of 83 Brucella and 2 Ochrobactrum strains based on 188 biochemical reactions tested with the Taxa Profile™ E plate. Hierarchical cluster analysis was performed by the Ward's linkage algorithm using the raw OD data.

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Additional file 7:

Metabolic activity of Brucella strains. Relative frequency (%) of positive and negative metabolic activity among 23 Brucella reference strains and 90 field isolates (Table 2) observed for the 93 substances tested in the Brucella specific Micronaut™ plate. Both quality and relative quantity are presented: - no metabolic activity (highlighted in green), + moderate metabolic activity (in orange), ++ strong metabolic activity (in red).

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Additional file 8:

Separation of Brucella spp. from clinically relevant bacteria. Relative frequency (%) of positive metabolic activity among Brucella and other bacteria observed for HP, Pyr-βNA (Pyr), urease, and NTA. Four enzymatic reactions which were revealed by the Micronaut-IDS database screening clearly discriminated Brucella from clinically relevant bacteria of other genera.

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