Table 5

Detection limits of BoNT DNA in crude toxin supernatants

Bot A

Bot B

Bot E

Bot F


Crude Toxin 2 ng

LOD

Crude Toxin 200 pg

LOD

LOD

Crude Toxin 20 pg

0.8 (LOD)

Crude Toxin 2 pg

Crude Toxin 200 fg

11.7

2.58

Crude Toxin 20 fg

29.2


LOD indicates the averaged limits of detection for that subtype in our mouse protection bioassay with identical serotypes used in toxin complex preparations.

Indicated in this table is the sensitivity of quantitative PCR for type-specific BoNT detection. Crude toxin supernatants with equivalent toxin protein amounts are listed. Values included in this table are the exact copy number of BoNT DNA detected in crude toxin preparations at the indicated amounts of protein. LOD indicates the averaged limits of detection for that subtype in our mouse protection bioassay with identical serotypes used in toxin complex preparations.

Hill et al. BMC Microbiology 2010 10:267   doi:10.1186/1471-2180-10-267

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