Table 4

Cross reactivity and specificity of primers and probes with all subtypes of C. botulinum Subtype Specific Primer and Probe set Used in Real Time PCR

BoNT subtype

strain

A

B

C

D

E

F

G


A1

Hall

+

-

-

-

-

-

-

A1

CDC 1757

+

-

-

-

-

-

-

A1

CDC 1744

+

-

-

-

-

-

-

A2

Kyoto-F

+

-

-

-

-

-

-

A2b

CDC 1436

+

+

-

-

-

-

-

A3

Loch Maree

+

-

-

-

-

-

-

B1

Okra

-

+

-

-

-

-

-

B1

CDC 1656

-

+

-

-

-

-

-

B1

CDC 1758

-

+

-

-

-

-

-

B2

213B

-

+

-

-

-

-

-

B2

CDC 1828

-

+

-

-

-

-

-

B3

CDC 795

-

+

-

-

-

-

-

B4 (npB)

Eklund 17B

-

+

-

-

-

-

-

Ba4

CDC 657

+

+

-

-

-

-

-

Bf

An436

-

+

-

-

-

-

-

C

Stockholm

-

-

+

-

-

-

-

C/D

6813

-

-

+

-

-

-

-

D

ATCC 11873

-

-

-

+

-

-

-

D

1873

-

-

-

+

-

-

-

D/C

VPI 5995

-

-

-

+

-

-

-

E1

Beluga

-

-

-

-

+

-

-

E2

CDC 5247

-

-

-

-

+

-

-

E2

CDC 5906

-

-

-

-

+

-

-

E3

Alaska E43

-

-

-

-

+

-

-

E4 (It butyr)

BL5262

-

-

-

-

+

-

-

F1 (prot)

Langeland

-

-

-

-

-

+

-

F2 (np)

Eklund 202F

-

-

-

-

-

+

-

F3 (baratii)

Orange

-

-

-

-

-

+

-

G

1354

-

-

-

-

-

-

+


"+/-" indicates amplification/no amplification by real time PCR

Indicated in the table are all strains tested with each type-specific BoNT primer and probe set. A (+/-) indicates amplification/no amplification by real time PCR.

Hill et al. BMC Microbiology 2010 10:267   doi:10.1186/1471-2180-10-267

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