Table 2

NTNH PCR detection in spiked healthy infant stool and a clinical infant botulism sample

Strain (genomic copies)

NTNH Detection


BoNT A (104)

++

BoNT A (102)

++

BoNT A (10)

+

BoNT A (1)

+

BoNT B (104)

++

BoNT B (102)

+

BoNT B (10)

+

BoNT B (1)

-

BoNT C (104)

++

BoNT C (102)

+

BoNT C (10)

+

BoNT C (1)

-

BoNT D (104)

++

BoNT D (102)

+

BoNT D (10)

-

BoNT D (1)

-

BoNT E (104)

++

BoNT E (102)

+

BoNT E (10)

+

BoNT E (1)

-

BoNT F (104)

++

BoNT F (102)

++

BoNT F (10)

+

BoNT F (1)

-

BoNT G (104)

++

BoNT G (102)

++

BoNT G (10)

+

BoNT G (1)

-

Clinical stool sample

++


++ Denotes strong PCR band

+ Denotes weak PCR band

- Denotes no PCR Band

Listed in this table are results from NTNH gene detection within spiked samples of a healthy infant stool sample as described in the materials and methods. Also included is the result from a confirmed case of infant botulism in California. (++) indicates a strong positive PCR product at the dilution tested, (+) is a weak positive PCR product, and (-) indicates no amplification detected.

Hill et al. BMC Microbiology 2010 10:267   doi:10.1186/1471-2180-10-267

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