Table 1

NTNH gene detection on C. botulinum and other clostridial strains

BoNT subtype

strain

PCR(DNA)a

(culture supernatant)b

other clostridia

strain

PCR(DNA)a


A1

Hall

+

+

C. absonum

ATCC 27555

-

A1

CDC 1757

+

+

C. baratii e

ATCC 27638

-

A1

CDC 1744

+

+

C. bifermentans

ATCC 638

-

A2

Kyoto-F

+

+

C. haemolyticum

ATCC 9650

-

A2b

CDC 1436

+

+

C. hastiforme

ATCC 25772

-

A3

Loch Maree

+

+

C. histolyticum

ATCC 19401

-

B1

Okra

+

+

C. novyi

ATCC 17861

-

B1

CDC 1656

+

+

C. novyi

ATCC 19402

-

B1

CDC 1758

+

+

C. novyi A

ATCC 19402

-

B2

213B

+

+

C. novyi B

ATCC 2706

-

B2

CDC 1828

+

+

C. perfringens A

ATCC 3624

-

B4 (npB)

Eklund 17B

+

+

C. perfringens A

ATCC 12915

-

Ba4

CDC 657

+

+

C. perfringens A

ATCC 12917

-

Bf

An436

+

+

C. perfringens A

ATCC 12918

-

C

Stockholm

+

-

C. perfringens A

ATCC 12919

-

C/D

6813

+

-

C. perfringens A

ATCC 13124

-

D

ATCC 11873

+

+

C. perfringens B

ATCC 3626

-

D

1873

+

nd

C. perfringens D

ATCC 3629

-

D/C

VPI 5995

+

+

C. perfringens D

ATCC 3630

-

E1

Beluga

+

-

C. perfringens D

ATCC 3631

-

E2

CDC 5247

+

nt

C. perfringens D

ATCC 12920

-

E2

CDC 5906

+

nt

C. perfringens E

ATCC 27324

-

E3

Alaska E43

+

+

C. ramosum

ATCC 25582

-

E4 (It butyr)c

BL5262

+

-

C. septicum

ATCC 12464

-

F1 (prot)

Langeland

+

+

C. sordelli

ATCC 9714

-

F2 (np)

Eklund 202F

+

-

C. sporogenes

ATCC 19404

-

F3 (baratii)d

Orange

+

nt

C. sporogenes

ATCC3854

-

G

1354

+

nd

C. subterminale

ATCC 25774

-

C. tertium

ATCC 14573

-

C. tetani

ATCC 10799

-

C. tetani

ATCC19406

-


a +/- indicates presence/absence of 101 bp band on agarose gel. Samples are purified DNA from bacterial cultures as described in the Methods section.

b Samples originate from filtered culture supernatants containing crude toxin. +/- indicates presence/absence of 101 bp band on agarose gel. nd = not detected, nt = not tested.

c BoNT E-producing strain of C. butyricum isolated from an infant case in Italy.

d BoNT F-producing strain of C. baratii.

eNon-toxin producing strain of C, baratii.

Results from conventional PCR detection of NTNH. A (+/-) indicates presence/absence of 101 bp band by agarose gel, respectively. DNA results indicate PCR detection of NTNH in purified DNA from both C botulinum and other Clostridial strains. Culture supernatant results indicate amplification of DNA within crude culture supernatants. NT indicates samples that were not tested.

Hill et al. BMC Microbiology 2010 10:267   doi:10.1186/1471-2180-10-267

Open Data