Open Access Research article

The liposoluble proteome of Mycoplasma agalactiae: an insight into the minimal protein complement of a bacterial membrane

Carla Cacciotto1, Maria Filippa Addis12, Daniela Pagnozzi2, Bernardo Chessa1, Elisabetta Coradduzza1, Laura Carcangiu1, Sergio Uzzau23, Alberto Alberti1* and Marco Pittau1

  • * Corresponding author: Alberto Alberti alberti@uniss.it

  • † Equal contributors

Author Affiliations

1 Dipartimento di Patologia e Clinica Veterinaria, Università degli Studi di Sassari, Sassari, Italy

2 Porto Conte Ricerche Srl, Tramariglio, Alghero (SS), Italy

3 Dipartimento di Scienze Biomediche, Università degli Studi di Sassari, Sassari, Italy

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BMC Microbiology 2010, 10:225  doi:10.1186/1471-2180-10-225

Published: 25 August 2010

Additional files

Additional file 1:

2-D PAGE map of liposoluble proteins from M. agalactiae PG2T illustrating the protein identifications obtained by MS on the 3-10NL pI Interval.

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Additional file 2:

2-D PAGE map of liposoluble proteins from M. agalactiae PG2T illustrating the protein identifications obtained by MS on the 7-11 pI Interval.

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Additional file 3:

2-D PAGE map of liposoluble proteins from M. agalactiae PG2T illustrating the protein identifications obtained by MS on the 4-7 pI Interval.

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Additional file 4:

Table listing all protein identifications obtained from 2-D PAGE maps. The proteins listed in this table were identified from 2-D PAGE maps of the M. agalactiae PG2T Triton X-114 fraction. Maps are represented in Additional files 1 (pH 3-10NL), 2 (pH 7-11) and 3 (pH 4-7).

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Additional file 5:

Protein profile of liposoluble proteins before and after precipitation. Right: approach used for GeLC-MS/MS characterization. The bars indicate the regions cut from the PAGE gel and subjected to mass spectrometry characterization. Protein identifications are reported in additional file 6, from top to bottom.

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Additional file 6:

Table listing all protein identifications obtained by GeLC-MS/MS of the M. agalactiae PG2T Triton X-114 liposoluble fraction. The protein profile used and the number of slices are reported in Additional file 5.

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Additional file 7:

Functional analysis, number of peptide hits, and method of detection of M. agalactiae PG2T liposoluble proteins. The results of 2D DIGE with the two field strains Nurri and Bortigali are also reported (TPH, total peptide hits; NA, not applicable).

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Additional file 8:

Proteins identified in the M. agalactiae proteome potentially resulting from Horizontal Gene Transfer events with M. mycoides subsp. mycoides and M. capricolum subsp. capricolum.

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Additional file 9:

Proteins identified in the M. agalactiae proteome potentially resulting from Horizontal Gene Transfer events with other bacteria.

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