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Open Access Research article

Matrix metalloproteinase-3 promoter polymorphisms but not dupA-H. pylori correlate to duodenal ulcers in H. pylori-infected females

Yi-Chun Yeh1, Hsiu-Chi Cheng23, Wei-Lun Chang23, Hsiao-Bai Yang45 and Bor-Shyang Sheu23*

Author Affiliations

1 Institute of Basic Medical Sciences, National Cheng Kung University Hospital, Medical College, Tainan

2 Institute of Clinical Medicine, National Cheng Kung University Hospital, Medical College, Tainan

3 Department of Internal Medicine, National Cheng Kung University Hospital, Medical College, Tainan

4 Department of Pathology, National Cheng Kung University Hospital, Medical College, Tainan

5 Department of Pathology, Ton-Yen General Hospital, Hsin-Chu, Taiwan

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BMC Microbiology 2010, 10:218  doi:10.1186/1471-2180-10-218

Published: 13 August 2010



This study investigated if the H. pylori dupA genotype and certain host single nucleotide polymorphisms (SNPs) of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs), including MMP-3, MMP-7, MMP-9, TIMP-1 and TIMP-2, might correlate with ulcer risk of H. pylori-infected Taiwanese patients.


Of the 549 H. pylori-infected patients enrolled, 470 patients (265 with gastritis, 118 with duodenal ulcer, and 87 with gastric ulcer) received SNPs analysis of MMP-3-1612 6A > 5A, MMP-7-181 A > G, MMP-9exon 6 A > G, TIMP-1372 T > C and TIMP-2-418 G > C by PCR-RFLP. The 181 collected H. pylori isolates were detected for the dupA genotype by PCR. The rates of dupA-positive H. pylori infection were similar among patients with duodenal ulcer (22.8%), gastric ulcer (20.0%), and gastritis (25.5%) (p > 0.05). Males had higher rates of duodenal ulcer and gastric ulcer than females (p < 0.01). Of H. pylori-infected patients, the MMP-3 6A6A genotype were more common in patients with duodenal ulcers than in those with gastritis (87.7% vs. 74.9%, p < 0.05) in females. This genotype had a 2.4-fold (95% CI: 1.02-5.66) increased risk of duodenal ulcer, compared to those with the 5A carrier. Combining the MMP-3/TIMP-1 genotype as 6A6A/CC, the risk of duodenal ulcer increased up to 3.6 fold (p < 0.05) in H. pylori-infected females.


The MMP-3 promoter polymorphism, but not the dupA-status, may correlate with susceptibility to duodenal ulcer after H. pylori infection in Taiwanese females.