Table 4

Primer sets used for qPCR analyses of the diel cycle of expression of selected genes from Prochlorococcus marinus PCC9511.

Locus tag

Gene

Forward Primer

Reverse Primer


RNA_42

rnpB

64-AAGTCCGGGCTCCCATATG

135-TGTGGCACTATCCTCACGGTTA

PMM0285

phrA

1000-GGAGAGACCGGAGTACCTATAGTTGA

1088-GCGACTATCATCCTACATCTATTATGCA

PMM0395

sepF

397-GAAAGAGTCGGTGAAAGCATTTTT

470-GCCTCCTCTGGGAAAGAACTAGT

PMM0565

dnaA

492-TGGTGTCGGTCTTGGAAAGAC

568-CTTTCGCATCTGGATCAATTTCT

PMM0937

umuC

762-TCAAGTAAGTAGAAGCTTTGGAAAACC

889-TAGTAATGGCAGATGATTTTAAGCTTTG

PMM1054

ruvC

331-GCAGGCTCTGGCAAAGCA

407-TTTGGTGCACGGGTTAAATTT

PMM1077

sasA

430-CTTTTAAGAATGGTTGCACATGAATT

511-TTTGTCCTAGTTTTTGACTTTGAATAGC

PMM1262

lexA

227-AGATCTTTGAGGGAGTCCCAATT

299-TGGAGGTCGGAAAATGTTTCA

PMM1309

ftsZ

415-GGGATAGTAACCAAGCCATTTTCA

494-TCTGCTAATCTTGCAATCCCTTCT

PMM1342

kaiC

666-AGGAACCGTACATATGAAAGGAGAA

746-CTCATCGCTCCTAAGGCAAAA

PMM1343

kaiB

142-AAACAACCTCAACTTGCTGAAGAA

193-TAACAGGAGGAGGTAAAATCTTTGC

PMM1452

atpD

435-AATCGACCCATCCCTCATTG

512-ATTTGAGAGGCAAGACTAGCATCA

PMM1455

atpH

68-GCCCGGGCCTTGGA

129-AGGTTGGCGGGCAATACC

PMM1562

recA

319-GCTGAACATGCTTTAGATCCAGTCT

398-GTATCTGGCTGCGAAACTAGTAAATTT

PMM1629

rpoD8

726-CAAAGCTGGGCAGCCAGTA

820-CAGTCCATTTCGATTTGCTCATC

PMM1645

mutS

2281-ACAAGAATTGGAGCCGTAGATGA

2366-TGATTTAGTATTGATGCTGTTTCTGACA

PMM1697

rpoD4

746-CTCAAAGTGCTCCATGCGC

848-GATTGTTCTATCCATCCCTTCCA

PMM1712

uvrA

2775-GATAATTGATCTTGGACCTGATGGA

2865-ACTTATTGGATGCTTCGCAACA


The locus tag of the corresponding genes in P. marinus MED4 are indicated

Kolowrat et al. BMC Microbiology 2010 10:204   doi:10.1186/1471-2180-10-204

Open Data