Table 4 |
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Primer sets used for qPCR analyses of the diel cycle of expression of selected genes from Prochlorococcus marinus PCC9511. |
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|
Locus tag |
Gene |
Forward Primer |
Reverse Primer |
|
|
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|
RNA_42 |
rnpB |
64-AAGTCCGGGCTCCCATATG |
135-TGTGGCACTATCCTCACGGTTA |
|
PMM0285 |
phrA |
1000-GGAGAGACCGGAGTACCTATAGTTGA |
1088-GCGACTATCATCCTACATCTATTATGCA |
|
PMM0395 |
sepF |
397-GAAAGAGTCGGTGAAAGCATTTTT |
470-GCCTCCTCTGGGAAAGAACTAGT |
|
PMM0565 |
dnaA |
492-TGGTGTCGGTCTTGGAAAGAC |
568-CTTTCGCATCTGGATCAATTTCT |
|
PMM0937 |
umuC |
762-TCAAGTAAGTAGAAGCTTTGGAAAACC |
889-TAGTAATGGCAGATGATTTTAAGCTTTG |
|
PMM1054 |
ruvC |
331-GCAGGCTCTGGCAAAGCA |
407-TTTGGTGCACGGGTTAAATTT |
|
PMM1077 |
sasA |
430-CTTTTAAGAATGGTTGCACATGAATT |
511-TTTGTCCTAGTTTTTGACTTTGAATAGC |
|
PMM1262 |
lexA |
227-AGATCTTTGAGGGAGTCCCAATT |
299-TGGAGGTCGGAAAATGTTTCA |
|
PMM1309 |
ftsZ |
415-GGGATAGTAACCAAGCCATTTTCA |
494-TCTGCTAATCTTGCAATCCCTTCT |
|
PMM1342 |
kaiC |
666-AGGAACCGTACATATGAAAGGAGAA |
746-CTCATCGCTCCTAAGGCAAAA |
|
PMM1343 |
kaiB |
142-AAACAACCTCAACTTGCTGAAGAA |
193-TAACAGGAGGAGGTAAAATCTTTGC |
|
PMM1452 |
atpD |
435-AATCGACCCATCCCTCATTG |
512-ATTTGAGAGGCAAGACTAGCATCA |
|
PMM1455 |
atpH |
68-GCCCGGGCCTTGGA |
129-AGGTTGGCGGGCAATACC |
|
PMM1562 |
recA |
319-GCTGAACATGCTTTAGATCCAGTCT |
398-GTATCTGGCTGCGAAACTAGTAAATTT |
|
PMM1629 |
rpoD8 |
726-CAAAGCTGGGCAGCCAGTA |
820-CAGTCCATTTCGATTTGCTCATC |
|
PMM1645 |
mutS |
2281-ACAAGAATTGGAGCCGTAGATGA |
2366-TGATTTAGTATTGATGCTGTTTCTGACA |
|
PMM1697 |
rpoD4 |
746-CTCAAAGTGCTCCATGCGC |
848-GATTGTTCTATCCATCCCTTCCA |
|
PMM1712 |
uvrA |
2775-GATAATTGATCTTGGACCTGATGGA |
2865-ACTTATTGGATGCTTCGCAACA |
|
|
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|
The locus tag of the corresponding genes in P. marinus MED4 are indicated |
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|
Kolowrat et al. BMC Microbiology 2010 10:204 doi:10.1186/1471-2180-10-204 |
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