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Open Access Highly Accessed Research article

Exocytosis and protein secretion in Trypanosoma

Anne Geiger1*, Christophe Hirtz23, Thierry Bécue2, Eric Bellard2, Delphine Centeno2, Daniel Gargani4, Michel Rossignol2, Gérard Cuny1 and Jean-Benoit Peltier2

Author Affiliations

1 UMR 177, IRD-CIRAD, CIRAD TA A-17/G, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France

2 INRA, UR1199, LPF; 2, place Pierre Viala - Bât. 13; 34060 Montpellier Cedex 01, France

3 UFR Odontologie, EA 4203, 545 Avenue du Pr Viala, 34193 Montpellier cedex 5, France

4 UMR, Biologie et Génétique des interactions Plante-Parasite, INRA, CIRAD, SUPAGRO, TA A54/K, Campus international de Baillarguet, 34398 Montpellier cedex 5, France

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BMC Microbiology 2010, 10:20  doi:10.1186/1471-2180-10-20

Published: 26 January 2010

Additional files

Additional file 1:

Table S1. Secreted proteins identified in 3 T. brucei gambiense strains separated on 1D gel. contains the identification of the proteins secreted by Biyamina (sheet 1), Feo (sheet 2), and OK strain (sheet 3) and their classification according to functional categories (MapMan bins nomenclature). For each protein, the number of matched peptides and the highest score are described.

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Additional file 2:

Table S2. Secreted proteins from OK strain identified on BN-PAGE gel. contains the list of the proteins identified in each spot (sheet 1), a nonredundant list of proteins classified according to their functional categories (sheet 2), and a nonredundant list of all the secreted proteins identified (BN+1D) in this study (sheet 3).

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Additional file 3:

Table S3. Secreted proteins from Leishmania donovanii and their corresponding Trypanosoma orthologs. contains the list of 358 proteins from L. donovanii identified in Silverman et al., 2008 [20] which were blasted against the T. brucei genome. The blast e scores > e-50 were reported as positive identification of T. brucei orthologs. Functional categories were assigned to L. donovanii-secreted proteins as well as the transmembrane span prediction (TMHMM) of these proteins.

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Additional file 4:

Table S4. Proteins identified in glycosome from T. brucei [19]. contains the list of 163 proteins from the glycosome proteome which were classified into functional categories (MapMan bins nomenclature).

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Additional file 5:

Table S5. Proteins identified in total proteome from T. brucei [18]. contains the list of 1071 proteins from the total proteome which were classified into functional categories (MapMan bins nomenclature).

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Additional file 6:

Table S6. Genome-wide prediction of secreted proteins using SignalP and secretomeP. contains the list of 1445 SignalP-predicted proteins (containing a putative transit peptide) from T. brucei and classified according to the number of predicted transmembrane spans (TMHMM prediction) (sheet 1). SecretomeP-predicted proteins from T. brucei were reported according to their p-value (sheet 2). The 3 highest classes p>0.9, 0.9>p>0.8, and 0.8>p>0.7 containing, respectively, 128, 583, and 875 proteins and their number of predicted transmembrane spans (TMHMM prediction) were reported.

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Additional file 7:

Table S7. Proteins identified in sucrose fractionated membranes from infected rat serum (IRS). contains the list of the IRS proteins. IRS proteins shared with ESPs or exosome are boxed in yellow and orange, respectively.

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Additional file 8:

Table S8. Additional informations on proteins identified in secretome. contains the list of the proteins identified in 1D and BN-PAGE gels spots. Protein score, number of peptides identified and number of peptides that fit to our stringent filter are provided.

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