Effect of carolacton on the comX-promoter activity of S. mutans. The CSP-induced comX-luc gene expression in the reporter strain construct was determined in the presence and absence of 2 μM carolacton. The luciferase activity was normalized against the optical density at 620 nm and measured for different time-points after induction of luciferase expression with 0.2 μM CSP. The expression of comX-luc in cultures which were not induced by externally added CSP and its inhibition by carolacton is also shown. Cultures were grown under anaerobic conditions as biofilms (A) or in suspension (B).
Kunze et al. BMC Microbiology 2010 10:199 doi:10.1186/1471-2180-10-199