Time course of biofilm growth of S. mutans in the presence and absence of carolacton. A Biofilm volume, determined by using only the SYTO9 (green) component of the LIVE/DEAD BacLight Bacterial Viability stain. Two representative experiments are shown. Green fluorescence, which is a measure of total biomass, is shown in absolute units. B Biofilm membrane damage, determined using the LIVE/DEAD BacLight Bacterial Viability stain. Green and red fluorescence was measured, and biofilm damage was calculated as reduction of the ratio of green/red fluorescence compared to controls without carolacton. Error values were calculated from the standard deviations of the green/red ratios of control and carolacton treated samples according to the error propagation formula of Gauss. Three representative experiments are shown. Biofilms were grown anaerobically. Mean and standard deviation are given for triplicate samples.
Kunze et al. BMC Microbiology 2010 10:199 doi:10.1186/1471-2180-10-199