Quantification of the viability of carolacton treated S. mutans biofilms determined by counting colony forming units (CFU) and by measuring membrane damage, calculated as the green/red ratio after LIVE/DEAD BacLight Bacterial Viability staining in percent of untreated controls. Biofilms were grown for 24 h under anerobic conditions. Each data point is the average +/- standard deviation of triplicate to fourfold determinations. The CFU in the control without carolacton was 2.1 × 107ml-1.
Kunze et al. BMC Microbiology 2010 10:199 doi:10.1186/1471-2180-10-199