Table 3

Detection of α-hly plasmid specific sequences in porcine STEC strains.

Size of PCR products with primersa


Serotype

No. strains

Plasmid group

1f+1r

32f + 32r

44 f + 44r

99 f + 99r


O138:H14b

4

3

678

2900

1500

650

O139:H1b

9

3

678

2900

1500

650

O139:H1b

1

single

678

2000

1500

650

O141:H4b

7

3

678

2900

1500

650

O141:H4b

3

2

678

2000

685

650

O2:H32c

1

2

678

2000

685

650

O36:H19c

1

2

678

2000

685

650


a) size of the PCR products (bp) with primers 1f/r, 32f/r, 44f/r and 99f/r as described in Tables 1+ 2. Digestion of PCR products by HinfI resulted in identical patterns between strains corresponding to the size of the PCR products obtained (Table 1)

b) Strains isolated from feces of diseased pigs [29]

c) Strains isolated from pork meat [29]

Burgos and Beutin BMC Microbiology 2010 10:193   doi:10.1186/1471-2180-10-193

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