Table 1

Relevant properties of strains carrying plasmid and chromosomally encoded α-hly determinants

PCR products with primers pairsa


strain

Serotypeb

Origin, referenced

hly-plasmid (kb)

Plasmid group

1f/r

(678 bp)

32f/r

(671 bp)

44f/r

(685 bp)

99f/r

(650 bp)

72f/r

(695 bp)

81f/r

(773 bp)


C4115

O26:[H11]

human, EPEC [21]

pEO5 (157)

1

+

+

+

+

-

-

TPE422

Or:H48

E. coli K12 (pEO5) [21]

pEO5 (157)

1

+

+

+

+

-

-

CB9866

O26:[H11]

cattle, EPEC [21]

pEO5 (157)

1

+

+

+

+

-

-

CB1027

O26:[H11]

human, EPEC [21]

pEO5 (157)

1

+

+

+

+

-

-

CB1030

O26:[H11]

human, EPEC [21]

pEO5 (157)

1

+

+

+

+

-

-

IP187

O26:[H11]

human, EPEC [21]

pEO5 (157)

1

+

+

+

+

-

-

84/2195

Ont:H10

dog [10]

pEO9 (146)

1

+

+

+

+

-

-

84-R

O121:H46

dog [10]

pEO13 (97)

1

+

+

+

+

-

-

374

Or:H48

mouse [24]

pHly152 (48)

2

+

e)

+

+

-

-

84-3208

O42:H37

dog, ETEC[10]

pEO11 (48)

2

+

e)

+

+

-

-

84-2573

O70:NM

dog, ETEC [10]

pEO12 (48)

2

+

e)

+

+

-

-

CB853

O138:H14

pig, STEC [29]

pEO853 (145)

3

+

f)

g)

+

-

-

CB855

O138:NM

pig, STEC [29]

pEO855 (140)

3

+

f)

g)

+

-

-

CB857

O157:NM

pig, ETEC [42]

pEO857 (97)

3

+

f)

g)

+

-

-

CB860

O149:H10

pig, ETEC [42]

pEO860 (48)

single

+

+

g)

+

-

-

84-2S

O75:H2

dog [10]

pEO14 (97)

single

-

-

-

-

-

-

536h

O6:K15:H31

human UPEC [20]

-

n.a

-

-

-

-

+

+

536-14

O6:K15:H31

PAI I deletion mutant of 536 [20]

-

n.a

-

-

-

-

+

-

695/83

O126:H27

human [19]

-

n.a

-

-

-

-

-

i)

J96h

O4:K6

human UPEC [46]

-

n.a

-

-

-

-

+

j)

KK6-16

E. cloacae

human [26]

-

n.a

k)

-

-

-

-

-


a) primer pairs and size of the PCR products obtained with strains TPE422 (pEO5) (primers 1f/r, 32f/r and 44f/r) and 536 (primers 81f/r and 72f/r) (see Table 2).

+ = a PCR product of the same size as obtained with strains TPE422 (pEO5) or 536, respectively.

- = no PCR product obtained

PCR products with other sizes than obtained with the reference strains are indicated for their length in bp.

b) O:H serotype, Or = rough LPS, Ont = O-antigen not typable, NM = non motile

c) α = alpha-hemolytic (α-hly genes), e-hly = enterohemolytic (EHEC-hly genes)

d) All strains were from feces if not otherwise indicated, ETEC = enterotoxigenic E. coli, STEC = shigatoxigenic E. coli, UPEC = uropathogenic E. Coli

e) 2000 bp PCR product (2007 bp as calculated from the nucleotide sequence of pEO11 [GenBank FM210249]

f) 2900 bp PCR product (2868 bp as calculated from the nucleotide sequence of pEO860) [GenBank FM210351]

g) 1500 bp PCR products

h) strain carries two α-hly determinants in the chromosome

i) 950 bp PCR product

j) 860 bp PCR product

k) 778 bp PCR product (calculated from nucleotide sequencing of KK6-16 [GenBank FM210352])

Burgos and Beutin BMC Microbiology 2010 10:193   doi:10.1186/1471-2180-10-193

Open Data