Trehalose accumulation by R. etli 12a3, R. gallicum bv. phaseoli 8a3, R. tropici CIAT 899, and R. leguminosarum bv. phaseoli 31c3. Cells were grown in their optimal minimal medium up to early stationary phase, and trehalose content was measured colorimetrically as described in Methods. For each strain, a growth curve under the same condition used to measure trehalose accumulation is shown. Histograms representing trehalose accumulation are place above the sampling time. Trehalose values shown are the mean of three replicas of each condition in two independent experiments ± SD (Standard deviation).
Fernandez-Aunión et al. BMC Microbiology 2010 10:192 doi:10.1186/1471-2180-10-192