Three signals were differentially produced in Xoo . rpfC mutant of Xoo strain was grown in YEB medium for 48 h and DSF-family signals were extracted and purified from the supernatants for HPLC analysis. The relative percentage of DSF, BDSF and CDSF in one sample was determined by the percentage of peak area in HPLC elute.
He et al. BMC Microbiology 2010 10:187 doi:10.1186/1471-2180-10-187