Biofilm formation by wild-type and isogenic mrk deletion strains. Strains were grown at 37°C under shaking conditions for 16 h in PVC microtitre plates containing M9 minimal medium, washed to remove unbound cells and stained with 0.1% crystal violet. Biofilm formation was quantified by resuspending adherent cells in ethanol-acetate (80:20) and measuring the absorbance at 595 nm. Shown are the results for E. coli MS2027, M184, ECOR15 and, ECOR28, K. pneumoniae M20, M124, M446, M542 and, M692, K. oxytoca M126 and, M239, C. koseri M546 and C. freundii M46 and their respective mrk deletion mutants.
Ong et al. BMC Microbiology 2010 10:183 doi:10.1186/1471-2180-10-183