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Open Access Highly Accessed Research article

A distinctive 'microbial signature' in celiac pediatric patients

Serena Schippa1*, Valerio Iebba1, Maria Barbato2, Giovanni Di Nardo2, Valentina Totino1, Monica Proietti Checchi1, Catia Longhi1, Giulia Maiella2, Salvatore Cucchiara2 and Maria Pia Conte1

Author Affiliations

1 Department of Public Health Sciences, 'Sapienza' University of Rome, Piazzale Aldo Moro 5, Rome, 00185, Italy

2 Department of Pediatrics, "Sapienza" University of Rome, Piazzale Aldo Moro 5, Rome, 00185, Italy

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BMC Microbiology 2010, 10:175  doi:10.1186/1471-2180-10-175

Published: 17 June 2010

Abstract

Background

Celiac Disease (CD) is an autoimmune disorder of the small intestine in which dietary gluten ingestion leads to a chronic enteropathy. Recently, scientific evidence suggested a potential role of gut microbiota in CD. To have a snapshot of dominant duodenal microbiota we analyzed the mucosa-associated microbiota of 20 children with CD, before and after a gluten-free diet (GFD) regimen, and of 10 controls. Total DNA was extracted from duodenal biopsies and amplification products of 16S ribosomal DNA were compared by temporal temperature gradient gel electrophoresis (TTGE). TTGE profiles were analyzed by statistical multivariate analysis.

Results

The average number of bands in TTGE profiles was significantly higher (P < 0.0001) in active (n.b. 16.7 ± 0.7) and inactive states (n.b. 13.2 ± 0.8) than in controls (n.b. 3.7 ± 1.3). Mean interindividual similarity index was 54.9% ± 14.9% for active disease, 55.6% ± 15.7% for remission state and 21.8% ± 30.16% for controls. Similarity index between celiac children before and after GFD treatment was 63.9% ± 15.8%. Differences in microbiota biodiversity were among active and remission state (P = 0.000224) and amid active CD and controls (P < 0.001). Bacteroides vulgatus and Escherichia coli were detected more often in CD patients than in controls (P < 0.0001).

Conclusions

Overall, the results highlighted a peculiar microbial TTGE profile and a significant higher biodiversity in CD pediatric patients' duodenal mucosa. The possible pathophysiological role of these microbial differences needs further characterization.