Table 4

Enzyme activity of enzymes and relative expression level of genes for acetate metabolism in pyruvate-grown cultures during phototrophic and chemotrophic growth.

Specific activity (nmole/min/mg protein)


Enzyme activity tested

Phototrophic growth

Chemotrophic growth

Relative gene expression level (light/dark)


acetyl-CoA synthetase (ACS)a

100 ± 20

N/A

45

acetate kinase (ACK)a

800 ± 40

600 ± 100

3

phosphotransacetylase (PTA)a

400 ± 50

500 ± 100

--


aActivity of ACS was determined by a colorimetric assay of PPi [39], and activity of ACK and PTA was determined by coupling assays reported previously [18].

Tang et al. BMC Microbiology 2010 10:150   doi:10.1186/1471-2180-10-150

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