Phototrophic growth of H. modesticaldum on pyruvate and various sugars, and mass spectra of (bacterio)chlorophylls extracted from cells grown on pyruvate and glucose. Growth of H. modesticaldum on 20 mM pyruvate, 40 mM sugars, or 0.02% yeast extract (A), and on 10 mM D-glucose, 40 mM D-glucose, or 40 mM 2'-fluoro-2'-deoxy-D-glucose (FDG) (B) as defined carbon source in the growth medium. Either no or only "vitamin-level" (0.02%) yeast extract is included in the growth medium, and detailed growth conditions are described in Materials and Methods. Mass spectra of (bacterio)chlorophylls extracted from cultures grown on pyruvate (I, upper panel) vs. [3-13C]pyruvate (II, lower panel) in PMS medium (C) and glucose (I, upper panel) vs. [U-13C6]glucose (II, lower panel) in YE medium (D).
Tang et al. BMC Microbiology 2010 10:150 doi:10.1186/1471-2180-10-150