Table 2

Effect of various carbon sources on AThTP production by different E. coli strains.

AThTP (pmol/mg of protein)


MG1655

Control

62 ± 6

D-Glucose (10 mM)

11 ± 2

L-Lactate (10 mM)

26 ± 8

Pyruvate (10 mM)

< 2

RelA-

Control

56 ± 12

D-glucose

< 2

SpoT-

Control

80 ± 6

D-Glucose

10 ± 3

CF5802

Control

62 ± 4

D-Glucose (10 mM)

< 2

CV2

Control

120 ± 11

D-glucose

< 2

L-lactate

< 2


an. d., not determined

The bacteria (A600 > 1) were incubated for 20 min at 37°C in minimal M9 medium containing substrates at the concentrations indicated. Mean ± SD for 3 - 9 experiments.

Gigliobianco et al. BMC Microbiology 2010 10:148   doi:10.1186/1471-2180-10-148

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