Table 1

Effect of various carbon sources on AThTP production in the BL21 E. coli strain.

AThTP(pmol/mg of protein)


Control

88 ± 6

D-Glucose (1 mM)

13 ± 4

D-glucose (2.5 mM)

9 ± 2

D-Glucose (5 mM)

< 2

D-Glucose (10 mM)

< 2

L-Lactate (10 mM)

14 ± 2

Succinate (10 mM)

6 ± 1

L-Malate (10 mM)

8 ± 2

D-Glucose (10 mM) + NaCl (1.2 M)

94 ± 13

D-Glucose (10 mM) + KCl (1.2 M)

92 ± 6

D-Glucose (10 mM) + Choline Cl (1.2 M)

131 ± 15

Streptomycina (10 μM)

62 ± 2

Neomycina (10 μM)

68 ± 3

AAb

12 ± 2

AAb + serine hydroxamate (0.5 mg/mL)

18 ± 2


aAll amino acids (40 μg/mL each) with the exception of serine

bNo carbon source present

The bacteria (A600 > 1) were incubated for 60 min at 37°C in minimal M9 medium containing substrates at the concentrations indicated. Mean ± SD for 3 - 9 experiments.

Gigliobianco et al. BMC Microbiology 2010 10:148   doi:10.1186/1471-2180-10-148

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