Inhibition of P. gingivalis biofilm formation by anti-HmuY IgG antibodies. P. gingivalis wild-type (A7436, W83, and ATCC 33277) strains and the hmuY deletion mutant strain constructed in A7436 (TO4) were grown in basal medium supplemented with hemin (Hm) or dipyridyl (DIP). The cells were washed with PBS, incubated with purified pre-immune or immune anti-HmuY IgGs, and inoculated into fresh media. The microtiter plate biofilms were stained with crystal violet. Data are shown as the mean ± SD of three independent experiments (n = 6). Differences between the cells incubated with pre-immune IgGs and cells incubating with immune anti-HmuY IgGs expressed as p values are given above the respective bars.
Olczak et al. BMC Microbiology 2010 10:134 doi:10.1186/1471-2180-10-134