Production of anti-HmuY antibodies in rabbits. The reactivity of serial dilutions of rabbit pre-immune and immune anti-HmuY (test I, test II, and immune-serum) sera with 100 ng per well HmuY immobilized on the microtiter plate (A) and the reactivity of pre-immune and immune anti-HmuY (test I, test II, and immune-serum) sera diluted 1:10,000 with varying amounts of HmuY immobilized in the wells of a microtiter plate (B) are shown. Data from three sera analyzed in triplicate are shown as the mean ± SD.
Olczak et al. BMC Microbiology 2010 10:134 doi:10.1186/1471-2180-10-134