Table 6

Primers used for Real-Time PCR

Target gene

Forward primer (5'-3')

Reverse primer (5'-3')

Product size (bp)

PCR Efficiency (%)

Correlation coefficient (R²)

Reference

Clostridium coccoides 16S

aaa tga cgg tac ctg act aa

ctt tga gtt tca ttc ttg cga a

440

97,8

0,998

[43]

Bifidobacterium 16S

cgc gtc ygg tgt gaa ag

ccc cac atc cag cat cca

244

93,0

0,995

[44]

Lactobacillus 16S

agcagtagggaatcttcca

caccgctacacatggag

341

98,6

0,998

[45,46]

Bacteroides spp.16S^a

cgg cga aag tcg gac taa ta

acg gag tta gcc gat gct ta

360

100,1

0,997

This study

Butyryl-Coenzyme A

gcn gan cat ttc acn tgg aay wsn tgg cay atg

cct gcc ttt gca atr tcn acr aan gc

530

97,5

0,965

[21]

V2-V3 16S region (HDA)^b

act cct acg gga ggc agc agt

gta tta ccg cgg ctg ctg gca c

200

113,7

0,991

[40]

^aThe bacteroides primer set was designed to amplify a segment of the DNA sequence represented by the highly homologous bands 4-7 in Table 5. ^bPCR for the HDA primer set was run in parallel for each set of primers for all samples.

Licht et al. BMC Microbiology 2010 10:13   doi:10.1186/1471-2180-10-13