Table 2

Effects of bacterial cell-derived immune elicitors on susceptibility of third-instar gypsy moth larvae reared without enteric bacteria (antibiotics) or with enteric bacteria (no antibiotics) to B. thuringiensis (Bt).

a) Bt cell preparation (DiPel, 50 IU)


Reared without antibiotics

Reared with antibiotics

Rearing treatment

Elicitor added to B. thuringiensis

Bt alone

Bt alone


No Antibiotics

Bt alone

--

< 0.0001

No Antibiotics

Enterobacter sp. NAB3

0.6882

< 0.0001

Antibiotics

Enterobacter sp. NAB3

0.0956

< 0.0001

No Antibiotics

Crude lipopolysaccharide

0.8231

< 0.0001

Antibiotics

Crude lipopolysaccharide

0.0001

0.4942

No Antibiotics

Purified lipopolysaccharide

0.7268

< 0.0001

Antibiotics

Purified lipopolysaccharide

< 0.0001

0.5731

No Antibiotics

Bacillus cereus peptidoglycan

0.0582

0.0100

Antibiotics

Bacillus cereus peptidoglycan

0.0065

0.7331

No Antibiotics

Vibrio fisheri peptidoglycan

0.1092

< 0.0001

Antibiotics

Vibrio fisheri peptidoglycan

0.0010

0.1276

No Antibiotics

Tracheal cytotoxin

0.0539

< 0.0001

Antibiotics

Tracheal cytotoxin

0.4070

< 0.0001

No Antibiotics

Lysozyme-digested V. fisheri peptidoglycan

0.2622

< 0.0001

Antibiotics

Lysozyme-digested V. fisheri peptidoglycan

0.2356

< 0.0001

No Antibiotics

Lysozyme-digested V. fisheri peptidoglycan + purified lipopolysaccharide

0.1120

< 0.0001

Antibiotics

Lysozyme-digested V. fisheri peptidoglycan + purified lipopolysaccharide

0.2328

0.0002


b) Bt Cry1Ac toxin (MVPII, 20 ug)


Reared without antibiotics

Reared with antibiotics


Rearing treatment

Elicitor added to B. thuringiensis

Bt alone

Bt alone


No Antibiotics

Bt alone

--

0.0202

No Antibiotics

Enterobacter sp. NAB3

< 0.0001

< 0.0001

Antibiotics

Enterobacter sp. NAB3

0.7182

0.0002

No Antibiotics

Crude lipopolysaccharide

0.6689

0.0919

Antibiotics

Crude lipopolysaccharide

0.0440

0.8517

No Antibiotics

Purified lipopolysaccharide

0.8138

0.0038

Antibiotics

Purified lipopolysaccharide

0.0456

0.5915

No Antibiotics

Bacillus cereus peptidoglycan

0.0651

< 0.0001

Antibiotics

Bacillus cereus peptidoglycan

0.0264

0.1951

No Antibiotics

Vibrio fisheri peptidoglycan

0.5111

0.0056

Antibiotics

Vibrio fisheri peptidoglycan

0.0196

0.8623

No Antibiotics

Tracheal cytotoxin

0.9977

0.0116

Antibiotics

Tracheal cytotoxin

0.0188

0.8914

No Antibiotics

Lysozyme-digested V. fisheri peptidoglycan

< 0.0001

< 0.0001

Antibiotics

Lysozyme-digested V. fisheri peptidoglycan

0.7613

0.0001

No Antibiotics

Lysozyme-digested V. fisheri peptidoglycan + purified lipopolysaccharide

0.0005

< 0.0001

Antibiotics

Lysozyme-digested V. fisheri peptidoglycan + purified lipopolysaccharide

0.5645

< 0.0001


Two formulations of B. thuringiensis, DiPel 50 IU (a) and MVPII 20 μg (b), were assayed. The significance (p-values) of the log-rank test comparing larval mortality of each experimental treatment group to Bt alone or Bt alone when reared with antibiotics is shown.

Broderick et al. BMC Microbiology 2010 10:129   doi:10.1186/1471-2180-10-129

Open Data