RealTime-PCR analysis of ldh gene as an internal control. Data presented here were generated from at least four separate sets of biofilm cultures and RealTime-PCR was carried out in triplicate and was repeated at least once. These data were normalized and further analyzed using a non-parametric Kruskal-Wallis Test and student t-test. The bar graphs represent the average (± standard deviation in error bars) of normalized copy numbers × (μg S. mutans total RNA)-1. No significant differences were observed between S. mutans grown in mono-species and those grown in dual-species biofilms. Abbreviations: Sm, S. mutans; Ss, S. sanguinis; So, S. oralis; Lc, L. casei, with Sm-Ss, Sm-So and Sm-Lc indicating dual-species biofilm of the selected bacteria.
Wen et al. BMC Microbiology 2010 10:111 doi:10.1186/1471-2180-10-111