Table 3

Strains and plasmids used in this study.

Strains or plasmids

Relevant characteristics

Reference or source


Strains

H. pylori

CCUG17874

wild-type strain

CCUG, Sweden

hp0256 KO

CCUG17874 Δhp0256::Cmr

This study

P1

wild-type strain

[57]

P79

P1 Strr

[58]

P79-hp0256KO

P79 Δhp0256::Cmr

This study

P79-0256/pIR203K04

P79 Δhp0256::Cmr with pIR203K04 (Kanr)

This study

P79-0256/pIR0601

P79 Δhp0256::Cmr with pIR0610 (Kanr)

This study

S. typhimurium

SJW1103

wild-type strain

[59]

MKM40

SJW1103 ΔfliJ

[59]

MKM40-pQE60

SJW1103 ΔfliJ with empty pQE-60

This study

MKM40-pQE60-0256

SJW1103 ΔfliJ with pQE-60-0256

This study

E. coli

One Shot TOP10

F- mcrA Δ(mrr-hsdRMS-mcrBC) ф80lacZ ΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG

Invitrogen, USA

Plasmids

pIR203K04

kanamycin resistance cassette (Kanr)

[51]

pIR0601

pIR203K04 with hp0256 gene under the control of hp0601 promoter

This study

C-term His-tagged expression vector (Ampr)

pQE-60

pQE-60 with hp0256 gene

Qiagen, Germany

pQE-60-0256

This study


Cm, chloramphenicol, Kan, kanamycin; Str, streptomycin

Douillard et al. BMC Microbiology 2010 10:106   doi:10.1186/1471-2180-10-106

Open Data