Table 3 |
||
|
Strains and plasmids used in this study. |
||
|
Strains or plasmids |
Relevant characteristics |
Reference or source |
|
|
||
|
Strains |
||
|
H. pylori |
||
|
CCUG17874 |
wild-type strain |
CCUG, Sweden |
|
hp0256 KO |
CCUG17874 Δhp0256::Cmr |
This study |
|
P1 |
wild-type strain |
[57] |
|
P79 |
P1 Strr |
[58] |
|
P79-hp0256KO |
P79 Δhp0256::Cmr |
This study |
|
P79-0256/pIR203K04 |
P79 Δhp0256::Cmr with pIR203K04 (Kanr) |
This study |
|
P79-0256/pIR0601 |
P79 Δhp0256::Cmr with pIR0610 (Kanr) |
This study |
|
S. typhimurium |
||
|
SJW1103 |
wild-type strain |
[59] |
|
MKM40 |
SJW1103 ΔfliJ |
[59] |
|
MKM40-pQE60 |
SJW1103 ΔfliJ with empty pQE-60 |
This study |
|
MKM40-pQE60-0256 |
SJW1103 ΔfliJ with pQE-60-0256 |
This study |
|
E. coli |
||
|
One Shot TOP10 |
F- mcrA Δ(mrr-hsdRMS-mcrBC) ф80lacZ ΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG |
Invitrogen, USA |
|
Plasmids |
||
|
pIR203K04 |
kanamycin resistance cassette (Kanr) |
[51] |
|
pIR0601 |
pIR203K04 with hp0256 gene under the control of hp0601 promoter |
This study |
|
C-term His-tagged expression vector (Ampr) |
||
|
pQE-60 |
pQE-60 with hp0256 gene |
Qiagen, Germany |
|
pQE-60-0256 |
This study |
|
|
|
||
|
Cm, chloramphenicol, Kan, kanamycin; Str, streptomycin |
||
|
Douillard et al. BMC Microbiology 2010 10:106 doi:10.1186/1471-2180-10-106 |
||
