Email updates

Keep up to date with the latest news and content from BMC Microbiology and BioMed Central.

Open Access Research article

Adhesion to and biofilm formation on IB3-1 bronchial cells by Stenotrophomonas maltophilia isolates from cystic fibrosis patients

Arianna Pompilio12, Valentina Crocetta12, Pamela Confalone12, Mauro Nicoletti1, Andrea Petrucca13, Simone Guarnieri4, Ersilia Fiscarelli5, Vincenzo Savini6, Raffaele Piccolomini12 and Giovanni Di Bonaventura12*

Author Affiliations

1 Department of Biomedical Sciences, "G. D'Annunzio" University of Chieti-Pescara, Abruzzo, Italy

2 Center of Excellence for Aging, "G. D'Annunzio" University Foundation of Chieti-Pescara, Abruzzo, Italy

3 Laboratory of Microbiology, "Sant'Andrea" Hospital, Rome, Italy

4 Department of Basic and Applied Medical Sciences, "G. D'Annunzio" University of Chieti-Pescara, Abruzzo, Italy

5 Clinical Microbiology Laboratory, Pediatric Hospital "Bambino Gesù", Rome, Italy

6 Clinical Microbiology and Virology, Department of Transfusion Medicine, "Spirito Santo" Hospital, Pescara, Italy

For all author emails, please log on.

BMC Microbiology 2010, 10:102  doi:10.1186/1471-2180-10-102

Published: 7 April 2010

Abstract

Background

Stenotrophomonas maltophilia has recently gained considerable attention as an important emerging pathogen in cystic fibrosis (CF) patients. However, the role of this microorganism in the pathophysiology of CF lung disease remains largely unexplored. In the present study for the first time we assessed the ability of S. maltophilia CF isolates to adhere to and form biofilm in experimental infection experiments using the CF-derived bronchial epithelial IB3-1cell line. The role of flagella on the adhesiveness of S. maltophilia to IB3-1 cell monolayers was also assessed by using fliI mutant derivative strains.

Results

All S. maltophilia CF isolates tested in the present study were able, although at different levels, to adhere to and form biofilm on IB3-1 cell monolayers. Scanning electron and confocal microscopy revealed S. maltophilia structures typical of biofilm formation on bronchial IB3-1 cells. The loss of flagella significantly (P < 0.001) decreased bacterial adhesiveness, if compared to that of their parental flagellated strains. S. maltophilia CF isolates were also able to invade IB3-1 cells, albeit at a very low level (internalization rate ranged from 0.01 to 4.94%). Pre-exposure of IB3-1 cells to P. aeruginosa PAO1 significantly increased S. maltophilia adhesiveness. Further, the presence of S. maltophilia negatively influenced P. aeruginosa PAO1 adhesiveness.

Conclusions

The main contribution of the present study is the finding that S. maltophilia is able to form biofilm on and invade CF-derived IB3-1 bronchial epithelial cells, thus posing a rationale for the persistence and the systemic spread of this opportunistic pathogen in CF patients. Experiments using in vivo models which more closely mimic CF pulmonary tissues will certainly be needed to validate the relevance of our results.