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Open Access Highly Accessed Research article

The analysis of oral microbial communities of wild-type and toll-like receptor 2-deficient mice using a 454 GS FLX Titanium pyrosequencer

Jongsik Chun12, Kap Y Kim3, Jae-Hak Lee2 and Youngnim Choi3*

Author Affiliations

1 School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, Republic of Korea

2 Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul, Republic of Korea

3 Programs in Oromaxillofacial Infection & Immunity and BK21 CLS, Seoul National University and Dental Research Institute, Seoul, Republic of Korea

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BMC Microbiology 2010, 10:101  doi:10.1186/1471-2180-10-101

Published: 6 April 2010

Abstract

Background

Although mice have long served as an animal model for periodontitis, information on the composition of their indigenous oral microbiota is limited. The aim of the current study was to characterize mouse oral bacterial flora by applying extensive parallel pyrosequencing using the latest model pyrosequencer, a Roche/454 Genome Sequencer FLX Titanium. In addition, the effect of Toll-like receptor (TLR) 2 deficiency on oral microbiota was evaluated.

Results

Eight oral bacterial communities of wild-type (n = 4) and TLR2 knock-out (n = 4) C57BL/6 mice were characterized by analyzing 80,046 reads of 16S rRNA genes obtained by pyrosequencing. Excluding the PCR primers, the average length of each sequencing product was 443 bp. The average species richness of the murine oral bacterial communities was estimated to be about 200, but the communities were dominated by only two main phyla and several species. Therefore, the bacterial communities were relatively simple. The bacterial composition of the murine oral microbiota was significantly different from that of humans, and the lack of TLR2 had a negligible effect on the murine oral microbiota.

Conclusion

Pyrosequencing using the Roche/454 FLX Titanium successfully characterized mouse oral bacterial communities. The relatively simple oral bacterial communities of mice were not affected by TLR2 deficiency. These findings will provide a basis for future studies on the role of periodontal pathogens in the murine model of periodontitis.