Table 3

List of phagosomal proteins identified by MS/MS post-infection with MAC 109 or 2D6 at different time points

MAC 109

2D6 mutant

Protein

Accession

(h)

(h)



Bacterial Uptake

number

0.5

4

24

0.5

4

24


Complement c1q tumor necrosis factor related protein 5

Q9BXJ0

x

x

x

Complement receptor 2

P20023

x

Macrophage receptor MARCO

Q9UEW3

x

x

x

Pulmonary surfactant protein D

P35247

x

Scavenger receptor with C type Lectin type 1

Q9BYH7

x

TNFRSF1A-associated via death domain/TRADD

Q15628

x

Tumor necrosis factor receptor member 1A/TNFRSF1A

1NCFA

x

Antigen Presentation & Recognition

Integrin alpha 1

P56199

x

Integrin alpha IIb

P08514

x

MHC class I

Q95ID4

x

MHC class II

I54427

x

Rab Interacting

EEA1

Q15075

x

Peroxin 5-related protein

Q81YB4

x

x

Rabaptin 5

Q15276

x

Cytoskeletal Proteins & Motors

Alpha actin

P62736

x

Ankyrin 1

Q6S8J3

x

Beta actin

Q96B34

x

x

E-MAP-115

Q14244

x

Keratin, type II cytoskeletal I

P04264

x

x

Kinesin/KIF3

O14782

x

Kinesin family member 26A

Q8TAZ7

x

L-plastin

P13796

x

x

Myosin heavy chain non-muscle type A

P35579

x

Myosin X with ATPase activity

Q9HD67

x

x

RHOC

P08134

x

TCP-1 zeta

P40227

x

Tubulin alpha

Q71U36

x

x

Tubulin alpha 2

Q13748

x

Tubulin alpha 3

Q71U36

x

Tubulin beta 2

P07437

x

x

Proteins in Biosynthetic Pathways

ABC transporter 2

Q9BZC7

x

ADP-ATP translocase

P05141

x

Aldoketo-reductase 3

Q9UFE1

x

ALG12

Q9BV10

x

ATP synthase

P06576

x

x

Fatty acid synthase

P49327

x

x

Fatty acid transporter member 1

Q6PCB7

x

GAPDH

P04406

x

Phosphatidyl inositol glycan class T

Q92643

x

IMP dehydrogenase

P12268

x

K-Cl co-transporter

T17231

x

Mitochondrial dicarboxylate carrier

Q9UBX3

x

Neutral amino acid transporter

Q15758

x

NUAK family, SNF1-like kinase 1

O60285

x

PI4KII

Q9BTU6

x

Pyruvate kinase

PS00110

x

x

x

Ribophorin II

Q5JYR7

x

Trehalose precursor

O43280

x

Transcriptional Regulators

Lysine-specific histone demethylase 1

O60341

x

CRSP complex subunit 2

O60244

x

CREB binding protein

Q75MY6

x

DEAH box polypeptide 9

Q08211

x

IFI 16

Q16666

x

Msx2-interacting protein

Q96T58

x

p-100 co-activator/SND1

Q7KZF4

x

p-300/CBP associated factor

Q92831

x

Zinc finger & BTB domain containing protein 4

Q9P1Z0

x

Zinc finger protein 588

Q9UII5

x

Zinc finger protein 43

P17038

x

60 S acidic ribosomal protein P2

P05387

x

60 S ribosomal protein L6

Q02878

x

x

60 S ribosomal protein L9

P32969

x

60 S ribosomal protein L14

P50914

x

Proteins Interacting with Signal Proteins

cAMP-specific 3',5'-cyclic phospho-diesterase

Q08493

x

Calcium & lipid binding protein/NLF2

388125

x

Chondroitin sulfate synthase 3

Q86Y52

x

CXC3C membrane-associated chemokine

P78423

x

Doublecortin & CaM kinase like-3

Q9C098

x

Dystrobrevin alpha

Q9Y4J8

x

Golgin subfamily A member 5

Q8TBA6

x

Microtubule associated-Ser/The kinase 3

O60307

x

Protein kinase A anchoring protein 9

Q99996

x

Protein kinase N

Q8NF44

x

Serine/theonine kinase 16

Q5U0F8

x

TER ATPase

P55072

x

Ion Channels

Amiloride-sensitive cation channel

Q96FT7

x

Voltage dependent-N-type calcium channel alpha 1B subunit

Q00975

x

Voltage dependent-T-type calcium channel alpha 1I subunit

Q9P0X4

x

Other Proteins

AFG3 like protein 2

Q9Y4W6

x

APBB1

O00213

x

Astrotactin 2

O75129

x

Apoptotic chromatin-condensation inducer in the nucleus

Q9UKV3

x

Clathrin heavy chain 1

Q00610

x

Ephrin B3

Q15768

x

48 kda histamine receptor subunit peptide 4

AAB34251

x

HSP 60

P10809

x

HSP 90

P07900

x

Importin alpha 2

P52292

x

Interferon regulatory factor 6

O14896

x

LRCH4/Ligand binding receptor

O75427

x

NADPH oxidase activator 1

Q2TAM1

x

Protein disulfide-isomerase A6

P07237

x

x

p-53-associated parkin-like cytoplasmic protein/PARC

Q8IWT3

x

Serine protease inhibitor

Q9NQ38

x

Vitrin

Q6UX17

x

14-3-3 protein/Tyrosine 3-monoxygenase

P62258

x

Proteins with Unknown Function

Hypothetical protein DKFZp434A128

T34567

x

Hypothetical protein LOC136288

Q8NEG2

x

Hypothetical protein KIA1783

Q96JP2

x

Hypothetical protein KIAA1783

Q96JP2

x

Hypothetical protein FLJ32795

Q96M63

x

Hypothetical protein FLJ42875

Q8N6L5

x

Hypothetical protein FLJ45491

Q6ZSI8

x

Hypothetical protein DKFZp434G131

Q9H0H4

x

Hypothetical protein FLJ46534

Q6ZR97

x

Hypothetical protein FLJ00361

Q8NF40

x


Complemented 2D6 mutant had similar results to the wild-type bacterium. Y = Yes; N = No

Jha et al. BMC Microbiology 2010 10:100   doi:10.1186/1471-2180-10-100

Open Data