Table 2

Macrophage genes with decreased expression in M. avium 109 but increased in 2D6 mutant 4 h post infection

Gene

Gene Bank ID

Name

Function

Fold induction (± SD)

p value <0.05


AMBP

X04494

Alpha-1-microglobulin

Negative regulation of immune response/Protease inhibitor

4.2 ± 0.7

Y

BLK

BC004473

B-lymphoid tyrosine kinase

Apoptosis

3.3 ± 0.3

Y

BMX

AF045459

BMX non-receptor tyrosine kinase

Intracellular signaling

18.6 ± 4.1

Y

CCR3

AF247361

Chemokine receptor 3

Signal transduction

4.1 ± 0.6

Y

CD53

BC040693

CD53 molecule

Growth regulation

4.1 ± 0.3

Y

CETN2

X72964

Centrin, EF-hand protein 2

Microtubule organization center

6.3 ± 0.9

Y

CHP

NP_009167

Calcium binding protein P22

Potassium channel regulator/Signal transduction

20.8 ± 3.5

Y

CR1

Y00816

Complement receptor 1

Bacterial uptake

4.3 ± 0.4

Y

CTSG

NM_001911

Cathepsin G

Bacterial killing

2.9 ± 0.2

Y

DCTN1

NM_004082

Dynactin 1

Lysosome and endosome movement

35.8 ± 8.0

Y

DDOST

D29643

Dolichyl-diphosphooligosaccharide-protein glycosyltransferase

N-linked glycosylation

3.3 ± 0.3

Y

DGKG

AF020945

Diacylglycerol kinase gamma

Intracellular signaling

5.3 ± 0.6

Y

DGKZ

U51477

Diacylglycerol kinase zeta

Intracellular signaling

48.1 ± 6.1

Y

EMILIN2

AF270513

Elastin microfibril interfacer 2

Cell adhesion

14.7 ± 3.7

Y

FOXC2

Y08223

Forkhead box C2

Transcription factor

5.9 ± 1.5

Y

GABBR1

Y11044

GABA receptor 1

Signal transduction

6.1 ± 2.0

Y

GABBR2

AF069755

GABA receptor 2

Signal transduction

2.8 ± 0.4

Y

GPR17

NM_005291

G-protein coupled receptor 17

Signal transduction

83.2 ± 12.5

Y

GZMA

NM_006144

Granzyme A

Apoptosis

2.1 ± 0.6

Y

IFNA1

NM_024013

Interferon alpha 1

Intracellular signaling

2.6 ± 1.0

Y

IL10RA

U00672

Interleukin 10 receptor alpha

Inhibition of proinflammatory cytokine synthesis

2.3 ± 0.2

Y

ITGB1

BC020057

Fibronectin receptor

Bacterial uptake

4.6 ± 0.7

Y

LCP2

NM_005565

Lymphocyte cytosolic protein 2

Immune response

37.5 ± 9.2

Y

MCAM

X68264

Melanoma cell adhesion molecule

Cell adhesion

4.7 ± 0.2

Y

MS4A1

M27394

Membrane-spanning 4-dmains

Immune response

9.6 ± 0.9

Y

PBX2

NM_002586

Pre-B-cell leukemia transcription factor 2

Transcriptional activator

3.0 ± 0.3

Y

PLTP

NM_006227

Phospholipid transfer protein

Lipid metabolism

3.6 ± 0.5

Y

RAB7

X93499

RAS related GTP binding protein

Vesicular transport regulation

3.4 ± 0.4

Y

RAB13

X75593

RAS related GTP binding protein

Small GTPase mediated signal transduction

7.5 ± 1.1

Y

RGS12

AF035152

Regulator of G-protein signaling 12

Negative regulation of G-protein signaling

3.0 ± 0.3

Y

RGS13

AF030107

Regulator of G-protein signaling 13

Negative regulation of G-protein signaling

2.6 ± 0.4

Y

S100A11

NM_005620

Calgizzarin

Motility, invasion & tubulin polymerization

9.6 ± 0.8

Y

TNFRSF17

Z29574

TNF receptor

Immune response

2.6 ± 0.3

Y

TUBB

AB062393

Tubulin beta

Microtubule based movement

4.0 ± 0.3

Y

YWHAZ

NM_003406

Tyrosine 3-monooxygenase

Signal transduction

4.3 ± 0.5

Y


Complemented 2D6 mutant had similar results to the wild-type bacterium. Y = Yes; N = No

Jha et al. BMC Microbiology 2010 10:100   doi:10.1186/1471-2180-10-100

Open Data