Effect of dsRNA on the cell fusion activity of RSV. A549 monolayers were transfected with 20 nM of anti-P or anti-F dsRNA and infected with RSV as described under Materials and Methods. At 40 h p.i., the monolayers were examined under a Nikon TS100F phase-contrast microscope at 40× magnification and digitally photographed with a Nikon Coolpix 995 camera. Note the syncytia in 'A', cytopathic effect without syncytia in 'B', and monolayers that appear unaffected and identical in 'C' and 'D'.
Bitko and Barik BMC Microbiology 2001 1:34 doi:10.1186/1471-2180-1-34