Email updates

Keep up to date with the latest news and content from BMC Microbiology and BioMed Central.

Open Access Research article

RNA triphosphatase is essential in Schizosaccharomyces pombe and Candida albicans

Yi Pei1, Beate Schwer2, Julia Saiz1, Robert P Fisher1 and Stewart Shuman1*

Author Affiliations

1 Molecular Biology and Cell Biology Programs, Sloan-Kettering Institute, New York, NY 10021, USA

2 Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10021, USA

For all author emails, please log on.

BMC Microbiology 2001, 1:29  doi:10.1186/1471-2180-1-29

Published: 20 November 2001

Abstract

Background

The first two steps in the capping of cellular mRNAs are catalyzed by the enzymes RNA triphosphatase and RNA guanylyltransferase. Although structural and mechanistic differences between fungal and mammalian RNA triphosphatases recommend this enzyme as a potential antifungal target, it has not been determined if RNA triphosphatase is essential for the growth of fungal species that cause human disease.

Results

We show by classical genetic methods that the triphosphatase (Pct1) and guanylyltransferase (Pce1) components of the capping apparatus in the fission yeast Schizosaccharomyces pombe are essential for growth. We were unable to disrupt both alleles of the Candida albicans RNA triphosphatase gene CaCET1, implying that the RNA triphosphatase enzyme is also essential for growth of C. albicans, a human fungal pathogen.

Conclusions

Our results provide the first genetic evidence that cap synthesis is essential for growth of an organism other than Saccharomyces cerevisiae and they validate RNA triphosphatase as a target for antifungal drug discovery.