Images of PCR amplification of the twenty-five minisatellites polymorphic in the Y. pestis strains DNA from three reference Y. pestis strains representing each of the main biovars, antiqua (lane 1), medievalis (lane 2) and orientalis (lane 3) and two Y. pseudotuberculosis strains (lanes 4 and 5) have been PCR amplified and an aliquot of the products has been run on 2% horizontal agarose gels as described. The length of the minisatellite motifs (U) and the size range is indicated on each panel. Yp2916ms07 has one of the shortest (10 bp) unit. Four alleles are clearly distinguished between the 150 and 200 bp marker fragments.
Le Flèche et al. BMC Microbiology 2001 1:2 doi:10.1186/1471-2180-1-2