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Open Access Highly Accessed Research article

Development of Dengue virus type 2 replicons capable of prolonged expression in host cells

Xiaowu Pang1, Mingjie Zhang1 and Andrew I Dayton12*

Author Affiliations

1 Laboratory of Molecular Virology, Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research, FDA, Bethesda, MD, USA

2 HFM 315 CBER/FDA, 1401 Rockville Pite, Rockville, MD 20852-1448, USA

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BMC Microbiology 2001, 1:18  doi:10.1186/1471-2180-1-18

Published: 24 August 2001

Abstract

Background

As part of a program to develop a Dengue virus vaccine which avoids the deleterious effects of antibody dependent enhancement (ADE) of infection mediated by antibodies to Dengue virus structural proteins, we have begun to investigate the possibility of designing Dengue vaccines based on non-structural proteins.

Results

Dengue constructs which lack major structural proteins replicate intracellularly in tissue culture. These replicons are capable of prolonged expression of Dengue virus non-structural proteins for at least seven days in culture.

Conclusions

Dengue virus genomes lacking major structural proteins can, like other flaviviruses, replicate intracellularly and express virus non-structural proteins with minimal toxicity to host cells. These findings pave the way for the development of dengue virus replicons as a form of live, attenuated virus vaccine.