Protein expression of IFIT-2 in RAW 264.7 macrophages. RAW 264.7 cells were stimulated with (A) IFN-β (50 U/ml), LPS (1 μg/ml), Pam3Cys (1 μg/ml) or IFN-γ (50 ng/ml), pretreated with GIR-208 or MAR1 prior to (C) LPS, (D) Pam3Cys or IFN-γ stimulation for indicated time periods and IFIT-2 and β-actin expression was detected by immunoblots. (B) RAW 264.7 cells were stimulated with or without IFN-β, staining was performed using anti-IFIT-2 and a secondary Cy2-labeled goat anti-rabbit antibody (green), Phalloidin-Tritc (red) and DAPI (blue) and detected by immunofluorescence. Big pictures; IFIT-2 expression, small pictures overlay of phalloidin and DAPI staining. At least three independent experiments with similar results with exception of B (two experiments).
Berchtold et al. BMC Immunology 2008 9:75 doi:10.1186/1471-2172-9-75