|
Physico-chemical properties of liposomes |
||
| Liposome type |
Mean Diameter |
Zeta Potential |
| nm ± SD(i) (%)(ii) |
mV ± SD(iii) |
|
|
|
||
| Entrapping DNA-hsp65 |
244.53 ± 64.05 (93.21) 985.92 ± 229.12 (6.79) |
32.8 ± 4.0 |
| Complexing DNA-hsp65 |
616.73 ± 152.35 (93.4) 2749.56 ± 774.90 (7.53) |
27.3 ± 2.3 |
| Empty |
247.47 ± 86.26 (93.96) 862.92 ± 140.78 (6.04) |
26.9 ± 2.4 |
|
(i) Mean ± standard deviation (SD). Liposome composition- EPC:DOTAP:DOPE 50:25:25 molar. Liposomes containing DNA-hsp65 at molar charge ratio 1:10 (cationic lipid:DNA). Diameter measurements were done in independent samples: n = 8 for liposomes complexed with DNA-hsp65 and n = 9 for liposomes entrapping DNA-hsp65 and empty liposomes. (ii) Percentages refer to distribution number-weighted of particles. (iii) Mean ± standard deviation (SD). Zeta potential measurements in three independent samples (n = 3) | ||
Rosada et al. BMC Immunology 2008 9:38 doi:10.1186/1471-2172-9-38 |
||