Table 1

Physico-chemical properties of liposomes

Liposome type

Mean Diameter

Zeta Potential

nm ± SD(i) (%)(ii)

mV ± SD(iii)


Entrapping DNA-hsp65

244.53 ± 64.05 (93.21)

985.92 ± 229.12 (6.79)

32.8 ± 4.0

Complexing DNA-hsp65

616.73 ± 152.35 (93.4)

2749.56 ± 774.90 (7.53)

27.3 ± 2.3

Empty

247.47 ± 86.26 (93.96)

862.92 ± 140.78 (6.04)

26.9 ± 2.4


(i) Mean ± standard deviation (SD). Liposome composition- EPC:DOTAP:DOPE 50:25:25 molar. Liposomes containing DNA-hsp65 at molar charge ratio 1:10 (cationic lipid:DNA). Diameter measurements were done in independent samples: n = 8 for liposomes complexed with DNA-hsp65 and n = 9 for liposomes entrapping DNA-hsp65 and empty liposomes.

(ii) Percentages refer to distribution number-weighted of particles.

(iii) Mean ± standard deviation (SD). Zeta potential measurements in three independent samples (n = 3)

Rosada et al. BMC Immunology 2008 9:38   doi:10.1186/1471-2172-9-38

Open Data