Cytokine production from the lung tissue of mice immunized with naked DNA-hsp65 or carried by liposome. Mice were immunized by intramuscular injection with naked hsp65 (400 μg) or by intranasal instillation with COMP-hsp65 (one dose of 25 μg). Thirty days after the last dose, mice were challenged intra-tracheally with M. tuberculosis H37RV and 30 days post infection, IFN-γ (A), IL-12 (B) and IL-10 (C) levels were determined by ELISA in lung homogenate. Data represent the mean ± SD of six mice per group of one of three independent experiments. *p < 0.01 were considered significant when compared to the saline group.
Rosada et al. BMC Immunology 2008 9:38 doi:10.1186/1471-2172-9-38