Figure 3.

Anti-hsp65 subtype antibody production in mice immunized with liposomes or naked DNA. BALB/c mice were immunized by intramuscular injection with 4 doses of 100 μg of naked DNA or a single dose of 100 μL of liposome (corresponding to 50 μg of DNA). Anti-hsp65 antibody levels were evaluated in mice serum by ELISA 30 days after the last immunization. A) IgG2a and B) IgG1 isotypes. Results are presented by mean ± SD of optical density. *p < 0.05 were considered significant when compared to saline group or their respective controls.